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Photosynthesis

Photosynthesis. 3.2 – Light Independent reaction. Settling activity. Using Fig 7 worksheet draw boxes around the 3 sets of reactions in the light dependent reaction. Learning outcomes. Success criteria. By the end of this lesson I can – Construct a diagram of the light independent reaction

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Photosynthesis

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  1. Photosynthesis 3.2 – Light Independent reaction

  2. Settling activity • Using Fig 7 worksheet draw boxes around the 3 sets of reactions in the light dependent reaction

  3. Learning outcomes Success criteria By the end of this lesson I can – Construct a diagram of the light independent reaction Answer exam questions on the work covered so far By the end of this lesson I will know – • The light-Independent reaction in detail • That carbon dioxide is accepted by ribulosebisphosphate (RuBP) to form 2 molecules of glycerate 3-phosphate • That ATP and reduced NADP are required for the reduction of GP to triose phosphate • That RuBP is regenerated in the calvin cycle • That triose phosphate is converted to useful organic substances

  4. Starter • Where does the light dependent reaction of photosynthesis take place? • Thylakoids (disc structures that are stacked together in groups called grana) • Name 2 ways chloroplasts structurally adapted to their function of capturing sunlight and carrying out the light dependent reaction. • Thylakoid membranes have a large surface area for the attachment of chlorophyll, electron carriers and enzymes • Proteins in the grana hold the chlorophyll to get optimum light absorption • Granal membranes have enzymes attached which help make ATP • Chloroplasts contain DNA and ribosomes so they can quickly manufacture proteins needed for the light dependent reaction

  5. Light independent reaction • What are the light dependent products? • ATP and reduced NADP • Where does the light independent reaction take place? • Stroma • How is the stroma adapted for this reaction? • Fluid contains enzymes that reduce carbon dioxide • Fluid surrounds grana so products of light dependent can diffuse through • Contains DNA and ribosomes so it can make proteins

  6. The Calvin cycle • Carbon dioxide diffuse through the stroma and dissolves into mesophyll cell walls, the membrane, cytoplasm, chloroplast membranes and stroma • Carbon dioxide is taken up by ribulosebisphosphate (RuBP), a 5-carbon compound. This gives an unstable 6-carbon compound, which quickly breaks down into 2 x 3-carbon glycerate 3-phosphate (GP) • This reaction is catalysed by the enzymes ribulosebisphosphatecarboxylase (rubisco). • Activated GP is reduced by (ATP and reduced NADP) into triose phosphate (TP) • NADP is reduced further going back into the light dependent reaction by accepting more H+ • Most Triose phosphate (TP) is use to regenerate RuBP using ATP • Some TP is converted to useful organic substances such as glucose

  7. The light-independent reactions of photosynthesis: Calvin Cycle CO2(1C) Rubisco Unstable intermediate (6C) RuBP Ribulose bisphosphate (5C) GP 2X Glycerate-3-phosphate (3C) ATP ADP ADP + Pi Reduced NADP TP 2X Triose phosphate (3C) ATP NADP Glucose(6C), amino acids and lipids

  8. Tasks • Complete the cut and stick of the calvin cycle using the information from the lesson. • Complete the exam paper question Homework – Complete exam questions

  9. Plenary Construct a simple overview of photosynthesis

  10. Learning outcomes Success criteria By the end of this lesson I can – Construct a diagram of the light independent reaction Answer exam questions on the work covered so far By the end of this lesson I will know – • The light-Independent reaction in detail • That carbon dioxide is accepted by ribulosebisphosphate (RuBP) to form 2 molecules of glycerate 3-phosphate • That ATP and reduced NADP are required for the reduction of GP to triose phosphate • That RuBP is regenerated in the calvin cycle • That triose phosphate is converted to useful organic substances

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