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Development of High (+)-Gossypol Stocks and Genetic Control of the Character

Development of High (+)-Gossypol Stocks and Genetic Control of the Character. Alois A. Bell, Robert D. Stipanovic, and Lorraine S. Puckhaber USDA-ARS-SPARC College Station, TX. O. O. O. C. H. O. H. C. H. O. H. O. H. H. C. H. O. O. H. H. O. H. O. O. H.

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Development of High (+)-Gossypol Stocks and Genetic Control of the Character

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  1. Development of High (+)-Gossypol Stocks and Genetic Control of the Character Alois A. Bell, Robert D. Stipanovic, and Lorraine S. Puckhaber USDA-ARS-SPARC College Station, TX

  2. O O O C H O H C H O H O H H C H O O H H O H O O H Gossypol Structure and Synthesis H O Hemigossypol Gossypol

  3. The (R)-Enantiomer of Gossypol[(+)-Gossypol Form]

  4. The (R)-Enantiomer of Gossypol[(+)-Gossypol Form] rotation around bond due to steric hindrance

  5. Effects of Gossypol in Poultry Diet * Mean Cumulative Weight Gain (MCWG; gm) and Cumulative Feed Conversion Ratios (CFCR) of 3-Wk-Old Broilers Fed Crushed Cottonseed (CCS) *From Bailey, C.A. et. al. J. Ag. Food Chem. 2000, 48, 5692-5695. † Means with no common letter within a column differ significantly (p ≤ 0.05).

  6. Selected Parents and Gossypol Contents Percentages of the Total Gossypol that Occurred as the (+)-Enantiomer in Seed and Flower Petals * Twenty seeds from a composite sample of several plants were ground together for seed determinations. †Determined for 1-6 separate flowers from single plants of each line.

  7. D r = 0.6214 r2 = 0.3861 C B A Distribution of (+)-Gossypol in BC1 (+)-Gossypol Percentages in Petals and Seed of (TX-2428 x Tamcot CAMD-E) x Tamcot CAMD-E Backcross Progeny

  8. r = 0.4005 r2 = 0.1604 D C B A Distribution of (+)-Gossypol in BC1 (+)-Gossypol Percentages in Petals and Seed of (TX-2443 x Tamcot CAMD-E) x Tamcot CAMD-E Backcross Progeny

  9. r = 0.81 r2 = 0.66 D C B A Distribution of (+)-Gossypol in BC1 (+)-Gossypol Percentages in Petals and Seed of (TX-2457 x Tamcot CAMD-E) x Tamcot CAMD-E Backcross Progeny

  10. r = 0.81 r2 = 0.66 D C B A Distribution of (+)-Gossypol in BC1 (+)-Gossypol Percentages in Petals and Seed of (TX-2457 x Tamcot CAMD-E) x Tamcot CAMD-E Backcross Progeny G+1G+2 G+2 G+1

  11. 30 C 68 B 76 D 80 A 63 25 20 Frequency 15 10 5 0 81-82 83-84 61-62 63-64 65-66 67-68 69-70 71-72 73-74 75-76 77-78 57-58 59-60 79-80 % (+)-Gossypol Frequency Distribution of (+)-Gossypol Percentages in Seed of BC1 Progeny and Median Values for Clusters A - D

  12. 30 A 64 B 70 C 79 D 86 25 20 Frequency 15 10 5 0 < 60 88-89 90-91 86-87 66-67 68-69 70-71 72-73 74-75 76-77 78-79 80-81 82-83 84-85 60-61 62-63 64-65 % (+)-Gossypol Frequency Distribution of (+)-Gossypol Percentages in Petals of BC1 Progeny and Median Values for Clusters A - D

  13. Segregation of (+)-Gossypol in PetalsBC2 Progeny (Suregrow 747)

  14. Segregation of (+)-Gossypol in PetalsBC2 Progeny (Stoneville 457)

  15. 100 90 80 % (+)-Gossypol 70 60 50 1 2 3 4 5 6 7 8 9 Plant Number Expression of G+1 Gene Alone in Petals(S1BC3 Progeny of 45-2-4)

  16. 100 90 80 % (+)-Gossypol 70 60 50 1 2 3 4 5 6 7 8 9 10 Plant Number Expression of G+2 Gene Alone in Petals(S1BC3 Progeny of 62-8-7)

  17. Expression of G+1 or G+2Alone in Petals and Seed

  18. 100 90 80 % (+)-Gossypol Seed 70 Petals 60 50 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Plant Number Expression of Combinations of G+1 and G+2 in Petals/Seed of Segregating Progeny(S1BC3 Progenyof47-8-1)

  19. Percentages of (+)-Gossypol Over Generations During the Development of Germplasm with Both G+1 and G+2

  20. Percentages of (+)-Gossypol in Progeny with Homozygous G+1 or G+2 Genes

  21. Combinations of G+1 and G+2 can be obtained in selfed progeny by combinations of petal and seed analyses. High (+)-gossypol lines that are homozygous and produce 93-95% (+)-gossypol in seed have been developed. The G+1 gene alone can increase the (+)-gossypol percentage to at least 90% in seed and is being evaluated as a single gene source of high (+)-gossypol. Conclusion

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