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Streptococcaceae II

Streptococcaceae II. Jeanne Filbey MT(ASCP) Division of Medical Technology Student Laboratory. Alpha-hemolytic streptococci. Streptococcus viridans group. Colony morphology Tiny, gray, domed, alpha hemolysis Optochin/Taxo P resistant Bile solubility negative Bile esculin negative

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Streptococcaceae II

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  1. Streptococcaceae II Jeanne Filbey MT(ASCP) Division of Medical Technology Student Laboratory

  2. Alpha-hemolytic streptococci Streptococcusviridans group • Colony morphology • Tiny, gray, domed, alpha hemolysis • Optochin/Taxo P resistant • Bile solubility negative • Bile esculin negative • No growth in 6.5% NaCl

  3. Alpha-hemolytic streptococci Streptococcusviridans group • Clinical significance • Normal flora • Generally non-pathogenic • Subacute bacterial endocarditis (#1 cause) • Liver abscesses, bacteremia • Streptococcus anginosus/milleri group • Deep wound infections

  4. Alpha-hemolytic streptococci Abiotrophia species • Nutritionally variant streptococci • Requires Vitamin B6 / pyridoxal to grow • Clinical significance • Normal flora • Endocarditis

  5. How can you differentiate Streptococcus pneumoniae from Streptococcus viridans group? Susceptibility to Optochin and bile solubility. Streptococcus pneumoniae is optochin sensitive and bile solubility positive. Streptococcus viridans group is optochin resistant and bile solubility negative.

  6. Streptococcus viridans group are clinically significant for what disease? Subacute bacterial endocarditis (#1 cause)

  7. Abiotrophia species are clinically significant for what disease? Subacute bacterial endocarditis

  8. Streptococcus-like bacteria Gemella species • Colony morphology • Resemble Streptococcus viridans group • Can be alpha or gamma hemolytic • Gram stain: GPC in pairs and chains • Easily decolorized • Pairs may have adjacent sides flattened

  9. Streptococcus-like bacteria Gemella species • Identification • PYR variable • Vancomycin sensitive • Glucose non “F” • Bile esculin negative • No growth in 6.5% NaCl

  10. Streptococcus-like bacteria Gemella species • Clinical significance • Normal flora of respiratory and GI tracts • Rarely isolated from humans • Opportunistic pathogen • Septicemia • Wounds, abscesses • Respiratory • UTI

  11. Streptococcus-like bacteria Leuconostoc species • Colony morphology • Resemble Streptococcus viridans group • Can be alpha or gamma hemolytic • Gram stain: GPC in pairs and chains • Can be coccobacilli in appearance

  12. Streptococcus-like bacteria Leuconostoc species • Identification • PYR negative • Vancomycin resistant • Glucose “F” • Bile esculin positive • Variable growth / no growth in 6.5% NaCl

  13. Streptococcus-like bacteria Leuconostoc species • Clinical significance • Normally found in environment • Opportunistic pathogen in immunosuppressed patients • Septicemia • Wounds • CSF

  14. Streptococcus-like bacteria Pediococcus species • Colony morphology • Resemble Streptococcus viridans group • Can be alpha or gamma hemolytic • Gram stain: GPC in pairs, tetrads and clusters

  15. Streptococcus-like bacteria Pediococcus species • Identification • PYR negative • Vancomycin resistant • Glucose “F” • Bile esculin positive • Variable growth / no growth in 6.5% NaCl • Group D antigen positive

  16. Streptococcus-like bacteria Pediococcus species • Clinical significance • Normally found in environment • Opportunistic pathogen in immunosuppressed patients • Blood • Saliva • Stool • Urine • Wounds

  17. Biochem: • G.S. • PYR • Van • Gluc • BE • 6.5NaCl • Gemella • Pair, chain • Variable • Sensitive • Non “F” • Negative • No growth • Leuconostoc • Pair, chain • Negative • Resistant • “F” • Positive • Variable • Pediococcus • Tetrad, cluster • Negative • Resistant • “F” • Positive • Variable • Type Grp D How can you differentiate Gemella, Leuconostoc and Pediococcus from each other?

  18. Summary… • Classification – hemolysis, C-substrate • Gram stain – GPC in pairs & chains • Identification • Beta Strep (PYR, Taxo A, SXT, Na hipp) • Alpha Strep (Optochin, bile solubility) • Gamma Strep (PYR, BE, 6.5% NaCl) • Strep-like organisms • Clinical significance of each organism

  19. Who am I? Gram stain BAP PYR Enterococcus species

  20. Who am I? Gram stain BAP PYR Streptococcus pyogenes

  21. Who am I? Gram stain BAP with optochin Streptococcus pneumoniae

  22. Who am I? Gram stain PYR Na hippurate BAP Streptococcus agalactiae

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