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Plaque Picking

Plaque Picking. We have viruses!!. Tucson High School Biotechnology Course Spring 2010. Results from Last Class. Synechococcus. Prochlorococcus. Results from Last Class: Controls. Negative Control. Positive Control 10 4 dilution (higher concentration of viruses). Positive

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Plaque Picking

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  1. Plaque Picking We have viruses!! Tucson High School Biotechnology Course Spring 2010

  2. Results from Last Class Synechococcus Prochlorococcus

  3. Results from Last Class: Controls Negative Control Positive Control 104 dilution (higher concentration of viruses) Positive Control 106 dilution (lower concentration of viruses

  4. Results from Last Class: Tara Oceans Samples No Plaques: 11 Tara Samples Plaques!! 6 Tara Samples

  5. Results from Tara Oceans

  6. Now What?? Pick Plaques! 3 μl No! Yes!

  7. What’s Next? Generate many more viruses We need a lot! Each virus: ~55 ag DNA (0.000000000000000055 g) We need: a few μg (0.000001 g) => 1.8 x 1010viruses (18000000000 viruses)

  8. A reminder Overlays are NOT solid! **Do NOT tilt, shake, jostle, or turn over your plates!**

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