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System Engineering M2D3

System Engineering M2D3. 11.03.09. Three part lab today. Part 1: finish Western Part 2: b -gal assay for your mutants Part 3: sequence analysis. Part 1: finish Western. Why are we performing a Western blot?. Part 2: b -gal assay. Miller units.

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System Engineering M2D3

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  1. System Engineering M2D3 11.03.09

  2. Three part lab today Part 1: finish Western Part 2: b-gal assay for your mutants Part 3: sequence analysis

  3. Part 1: finish Western Why are we performing a Western blot?

  4. Part 2: b-gal assay Miller units Why are we testing both light and dark grown cells? Should we test + and – HA tag? How many replicates should we test?

  5. Part 3: Sequence analysis Retrieve the sequences find the reverse complement BLAST your sequence (= query) vs pCph8 (= subject) Will pCph8 have HA tag? What will you do with your amino acid changes (if you have any)?

  6. Part 3: Sequence analysis

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