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Amy Alter Erika Pike Lake Shore High School

The Affect of goldfish (Carassius auratus) on the Percent of Chloroplasts in Elodea Densa as Determined by Differential Centrifugation. Amy Alter Erika Pike Lake Shore High School. Background Information. Elodea densa Water plant. Carassius auratus Common goldfish.

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Amy Alter Erika Pike Lake Shore High School

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  1. The Affect of goldfish (Carassius auratus) on the Percent of Chloroplasts in Elodea Densa as Determined by Differential Centrifugation Amy Alter Erika Pike Lake Shore High School

  2. Background Information • Elodea densa • Water plant • Carassius auratus • Common goldfish

  3. Background Information • Nitrogen cycle NO2 Nitrite Fish Waste Nitrate Buildup NO3 Nitrate NO3 Ammonia Plant Waste Plant Fertilizer

  4. Problem Statement • How does the presence of a goldfish (Carassius auratus) affect the percent of chloroplasts in Elodea densa?

  5. Hypothesis • If a goldfish (Carassius auratus) is placed with Elodea (Elodea densa), then the elodea will have more chloroplasts than elodea grown without a goldfish

  6. 60 Goldfish 84 Regular-Quart Mason Jars 150 Pieces of Elodea densa Centrifuge 0.5M Sucrose Solution Metric Balance Mortar Pestle Cheese Cloth Glass Funnel Centrifuge Tubes Sharp Sand 25mL-Graduated Cylinder 10mL-Graduated Cylinder Materials

  7. Goldfish

  8. 60 Goldfish 60 Regular-Quart Mason Jars 150 Pieces of Elodea densa Centrifuge 0.5M Sucrose Solution Metric Balance Mortar Pestle Materials

  9. Mortar and Pestle

  10. Cheese Cloth

  11. Glass Funnel

  12. 60 Goldfish 60 Regular-Quart Mason Jars 150 Pieces of Elodea densa Centrifuge 0.5M Sucrose Solution Metric Balance Mortar Pestle Cheese Cloth Glass Funnel Centrifuge Tubes Sharp Sand 25mL-Graduated Cylinder 10mL-Graduated Cylinder Materials

  13. 10mL-Graduated Cylinder

  14. ProcedureJar Preparation • Label 30 jars with “Control” and the jar number, 30 jars with “Experimental” and the jar number, and 24 jars with “Experimental extra” and the jar number • Dechlorinate tap water following the directions on the bottle of Insto-Chlor and add 1 quart (800mL) of water to each jar. • Mass out approximately 4.0g of Elodea and place in all jars • Mass out the goldfish, place goldfish weighing between 1.0 and 2.0 grams in all 30 of the experimental jar and all 24 of the experimental extra jars • Place all 60 jars near a light source, such as under a light or near a window.

  15. Procedure • Place centrifuge tubes, graduated cylinders, and sucrose solution in an ice bath to keep them cold • Mass the test tube to be used in the trial • Grind plant into a paste

  16. Procedure • Slowly add sucrose solution

  17. Procedure • Filter the pulp into centrifuge tube

  18. Procedure • Place the centrifuge tube in the centrifuge • Spin for five minutes • Decant the top 5mL • Spin for fifteen minutes • Pour off any remaining liquid

  19. Procedure • Mass the test tube with the chloroplast pellet • Subtract the mass of the test tube from the mass of the test tube with the pellet to find out the weight of the chloroplast pellet

  20. Data and Observations • Day 7: all Experimental and Control jars moved • After day 11, when fish died the jar was removed and replaced by an experimental extra jar • All fish were fed approximately every other day

  21. Data and Observations • Plants in experimental jars were greener and crisper than those in the control jars • The pulp was also greener that the pulp of the control jars

  22. Data Analysis and Interpretation

  23. t=((mean1)(mean2))/√(((SD1)2/N1)((SD2)2/N2)) Our Formula t=((6.07)(4.97))/√(((4.31)2/30)((1.68)2/30)) null hypothesis (H0) µ1= µ2 Alternative hypothesis (Ha) µ1< µ2 Data Analysis and Interpretation

  24. Error • Lack of time • Bacteria did not build up to complete the nitrogen cycle • Ammonia build-up • Insufficient light • Centrifuge speed • Fish are not predictable • Feeder goldfish

  25. Conclusion • How does the presence of a goldfish (Carassius auratus) affect the percent of chloroplasts in Elodea densa? • If a goldfish (Carassius auratus) is • placed with Elodea (Elodea densa), then • the elodea will have more chloroplasts • than elodea grown without a goldfish REJECTED

  26. Real Life Applications • Low-maintenance planted aquariums • Nitrogen Cycle • Fertilizers • Goldfish’s ability to survive in small environments

  27. Further Experimentation • Different fish with the same set-up • Betta splenden • Larger containers • Filtration on containers • Different comparisons • Fertilizers • Fish

  28. Acknowledgements • Ms. DeMaria • Mrs. Moore • Ms. Pike • Cynthia Anthony • Mr. and Mrs. Schroeder • Mr. Supal • Mr. Estapa

  29. Picture Acknowledgements • http://www.dewinters.nl/english/fishes/Betta%20splendens.htm • http://www.goldfishutopia.net/gallery/kablah/ • http://www.petsmart.com/global/product_detail.jsp?PRODUCT%3C%3Eprd_id=845524441776829&FOLDER%3C%3Efolder_id=2534374302030112&ASSORTMENT%3C%3East_id=2534374302023693&bmUID=1104255833546 • http://www.hoftiezer.net/personal/aquaria/images/20030428_front_04_small.jpg • http://www.bigalsonline.com/catalog/product.xml?product_id=28603&category_id=3349&pcid1= • http://www.hebs.scot.nhs.uk/library/img/chiMaA01.jpg

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