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LC-MS for drugs of abuse and related compounds

LC-MS for drugs of abuse and related compounds. 毒物分析. 授課教師：賴滄海教授 04-01-2010. Range of application. Schematic diagram of the general instrument setup of LC equipment. Van Deemter Equation. H = A + B/v + Cv H = L/N H: Height equivalent of theoretical plates L: Column length

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LC-MS for drugs of abuse and related compounds

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  1. LC-MS for drugs of abuse and related compounds 毒物分析 授課教師：賴滄海教授 04-01-2010

  2. Range of application

  3. Schematic diagram of the general instrument setup of LC equipment

  4. Van Deemter Equation • H = A + B/v + Cv H = L/N H: Height equivalent of theoretical plates L: Column length N: Number of theoretical plates

  5. Figure 3.2

  6. Electrospray Ions Nebulizer (gas shown in red) Heated nitrogen drying gas -4000 V Solvent spray  +   +              Dielectric capillary entrance Evaporation Rayleigh Limit Coulomb Explosion Evaporation Analyte Ion API-Electrospray Ionization

  7. API-Electrospray Ion Source High Voltage Mesh Assembly

  8. Nebulize • Evaporate Droplets • Gas-phase ionization [Solvent + H]+ + A  Solvent + [A + H]+ APCI Ionization Process

  9. APCI Ion Source Corona Needle

  10. Mass resolution 2000 and 2001 Resolution = 2000/1 = 2000 Mass accuracy (ppm) [Theoretical mass (180.0935924) – observed mass (180.094)/ Theo mass (180.0935924)] = - 2.3 ppm

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