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Calculating the net charge on an amino acid. pKa 2. pKa = 9. First, identify the ionizable groups (click for the answer). charge = +1. pH = pKa therefore, 50% ionized. pKa = 9. pKa =2. pH = 2. What happens at pH 2? (click to see if you’re correct). charge = +1. 50% ionized
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pKa 2 pKa = 9 First, identify the ionizable groups (click for the answer)
charge = +1 pH = pKa therefore, 50% ionized pKa = 9 pKa =2 pH = 2 What happens at pH 2? (click to see if you’re correct)
charge = +1 50% ionized 50% unionized ionized charge = -1 unionized charge = 0 Average = -0.5 ionized charge = -1 unionized charge = 0 Average = -0.5 ionized charge = -1 unionized charge = 0 Average = -0.5 Net charge = (+1) + (-0.5) = +0.5