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Lab 7, PCA of small data set Coffeomics , groups 1 and 2

Lab 7, PCA of small data set Coffeomics , groups 1 and 2. Nils Nyberg NPR, Department of Drug Design and Pharmacology. Quality control of coffee. Check the differences and similarities on normal black coffee and Espresso 600 µL Coffee + 50 µL 1 M phosphate buffer ( pD 7.4), D 2 O

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Lab 7, PCA of small data set Coffeomics , groups 1 and 2

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  1. NTDR, 2012 Lab 7, PCA of small data set Coffeomics, groups 1 and 2 Nils Nyberg NPR, Department of Drug Design and Pharmacology

  2. Quality control of coffee • Check the differences and similarities on normal black coffee and Espresso • 600 µL Coffee + 50 µL 1 M phosphate buffer (pD 7.4), D2O • 4 × 4 = 16 samples, analyzed twice = 32 spectra • Water signal suppression by presaturation

  3. “Special coffee”-buttons • What’s the secret formula of the special coffee-selections? • 600 µL Coffee + 50 µL 1 M phosphate buffer (pD 7.4), D2O • Centrifuge • 4 × 2 = 8 samples, prepared in duplicates = 16 tubes • Each tube analyzed twice = 32 spectra • Water signal suppression by presaturation

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