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Dr. Borislav ANGELOV

Large channels in cationic PEGylated cubosomes. Dr. Borislav ANGELOV. Supramolecular Polymer Systems I nstitute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic. L. D w. Large membrane proteins need larger channels for crystallization. D24, LURE Time Resolved SAXS.

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Dr. Borislav ANGELOV

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  1. Large channels in cationic PEGylated cubosomes Dr. Borislav ANGELOV Supramolecular Polymer Systems Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic

  2. L Dw Large membrane proteins need larger channels for crystallization D24, LURE Time Resolved SAXS Unit Cell a LCP unit cell Bilayer Thickness L 3 nm 7 nm Diameter of Water channels

  3. PEGylation leads to 4 times larger channels in the cubic nanoparticles J.Phys.Chem.Lett. 2013‏ Soft Mater 2015

  4. Historical Notes 1968 V. Luzzati reported micellar lipid cubic phase 1983 W. Longley and T. J. McIntosh reported diamond type bicontinuous cubic phase 1984-88 J. Friedel, J. Charvolin, and J.-F. Sadoc suggested a model for the formation of cubic phase based on proliferation of disclinations in lamellar phase 1989 K. Larsson outlined two application of cubic phase: (i) protein ordering and (ii) nanoparticle dispersion 1994 T. Landh reported cubic phase with large channels 1996 E. M. Landau and J. P. Rosenbusch crystallized membrane protein in cubic phase

  5. PEG & Ω3 derivatives induce structural intermediates of vesicle to cubosome transition Formation of a tetrahedral channel from vesicles Langmuir, 2012‏

  6. Cubosome with 10 nodes Model SAXS Experimental cryo-TEM Langmuir, 2012‏

  7. Cubosomenanoparticle growth

  8. a NPs + -chymotrypsinogen A lg I (a.u.) NPs lg I (a.u.) q(Å-1) q (Å-1) Protein entrapment in PEGylated lipid hexosomes MO/ Ω3 /VitE/PEG1000+chymotrip. Int. J. Pharm, 2013

  9. Millisecond Dynamics of Protein Loading in Lipid Nanocarriers ID02 beamline Rapid-mixing stopped-flow device coupled to real-time SAXS measurements Angelov et al., Acs Nano, 2014, 8, 5216-5226

  10. BDNF Protein Loading Creates Multiphase Cubic Lipid Nanocarriers Three final curvatures different from the initial Protein-induced ordering and cubic domains inside the Particle

  11. Time Dependence of Protein Loading in Lipid nanocarriers 82% upload within ms

  12. Stopped Flow pDNA/Lipid Assembly Kinetics Angelov, B, Angelova, A, et al., J.Phys.Chem.Lett., 2013, 4, 1959‏

  13. Time Constant of the Kinetics 14

  14. Perspectives Large channels formation Electro formation of large channels When the LCP Screening kit will be delivered ? Stability under loading: Loading changes the structure

  15. Collaborations Vasyl Garamus Angelina Angelova Markus Drechsler www.mardre.de Sergey Filippov Petr Stepanek

  16. Thank You for Your Attention!

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