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Investigating the growth of high-temperature microbes in specially designed media similar to those found in Yellowstone's hot springs for potential applications on Europa and Mars. The project involves culturing microbes from hot spring samples in synthetic media, with laboratory experiments based on geochemical data. Results will help identify, isolate, and characterize unique microorganisms adapted to extreme environments. Join us in exploring this exciting research journey!
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Growing High Temperature Microbes in Geochemically Designed Media Selisa Rollins Mentor: Dr. Everett Shock Arizona State University
Why Yellowstone? • Water • Warm • Easy access to microorganisms that do not use photosynthesis.
Geochemical Data • pH=7.678 • Temperature=79.4°C • Conductivity=2900μS
Experimental Design Representative Geochemical Data Laboratory
Culturing Microbes Inoculate Synthetic Hot Spring Media with Hot Spring Biomass Prepare Test Tubes Containing Media Place Tubes in 80°COven
Summary and Plans • Transfer sustained microbes • Isolate a microbe • Characterize microbe + represents # of microbes per screen view