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Extraction of DNA

Extraction of DNA. Lab #9. Step 1: Extract DNA from Banana. Follow the instructions listed on your handout. (Steps # 1-9) Materials on the cart : Amt you need -Shampoo 1 teaspoon -Salt 2 pinches -Vial w/chilled 91% alcohol 1 vial/pair

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Extraction of DNA

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  1. Extraction of DNA Lab #9

  2. Step 1: Extract DNA from Banana • Follow the instructions listed on your handout. (Steps # 1-9) • Materials on the cart: Amt you need -Shampoo 1 teaspoon -Salt 2 pinches -Vial w/chilled 91% alcohol 1 vial/pair -De-ionized water 20 ml -Banana mixture 3 teaspoons **Important notes & Reminders: -Note the appearance & describe what you see. -Rinse out your vials after you have made your observations -Answer questions # 1-4

  3. Step 2: Extracting Your DNAhttp://www.pbs.org/wgbh/nova/teachers/activities/2809_genome.html Supplies are in the back of the room. 1) Pick up a dixie cup w/ salt water (2 tsp) 2) Swish around in your mouth thoroughly for 30 sec. 3) Spit back into the cup 4) Pour the contents of the cup into the vial with soap solution. 5) Gently rock the vial back & forth (3 min) 6) Take one pipette full of chilled alcohol and slowly add it to the vial while tilted making sure that they don’t mix. 7) Let stand for 1 minute 8) Gently mix the alcohol into the soap layer with a glass rod. 9) DNA should precipitate (whitish) –Be patient

  4. Final Steps 10) Collect your DNA using a pipette or stirring rod and place into the capped containers. Add alcohol to preserve it. 11) Seal and secure with glue. 12) Write your initials on container (very small) • Place your DNA in the container w/your period • Pick up your DNA at lunch 15) Clean up: Rinse out the vials Throw out paper cups **Please refill your vial with one pipette full of soap and place it back in the rack before you leave. Thanks & have a Great Thanksgiving!!!

  5. Summary of Results -Due Friday Dec. 2 • Title • Introduction/Background • Separation of DNA: Explain the process -Why does this technique work? -Why do you need salt, shampoo, & alcohol? • Results: Explain what you observed • Describe what you saw • How was the Banana DNA different from your DNA? • How could you verify that what you have is DNA? • Conclusion/Evaluation: • How could this lab activity be improved? (Sources of error) • What else could be used? Other techniques?

  6. Upcoming Labs • Lab #10: Bacterial Transformation (done in class) • Lab #11: Analysis of DNA w/ Gel Electrophoresis *Both are AP recommended labs and must be done. **Important note: Lab #11 will require extra time to do. -This lab will be done after school on TWO days -You will choose which day you can come. (sign up list) -The time needed to do the lab: 2 hrs -Please let your parents know about this upcoming lab Possible Days to choose from: 1st Day: Mon. December 5th 3:30pm to 5:30pm 2nd Day: Wed. December 7th 3:30pm to 5:30pm

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