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Analysis and Sorting of T reg Cells on the FACSAria. John Daley Director of Flow Cytometry Hematologic Neoplasia Dana-Farber Cancer Institute Harvard Medical School. Picture credit:. http://www.bdbiosciences.com/features/products. From BD Biosciences Website Featured products:
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Analysis and Sorting of T reg Cells on the FACSAria John Daley Director of Flow Cytometry Hematologic Neoplasia Dana-Farber Cancer Institute Harvard Medical School Picture credit: http://www.bdbiosciences.com/features/products
From BD Biosciences Website Featured products: Regulatory T cells http://www.bdbiosciences.com/features/products /display_product.php?keyID=162
The way we used to sort Human CD4+CD25+ PBL’s on the FACSAria SORT TOP 2- 3% CD25 HI
Four way reg sort Multicolor(5) Minor populations : Four way sorting: Treg Story CD45ra FITC CD127 PE CD25 PE-CY5 CD4 PE-CY7 CD3 PAC BLUE
Compensation Comparisons • Manual Comp with cells and actual reagents • Autocomp with cells and actual reagents • Autocomp with beads and actual reagents • Autocomp with beads and CD45+ reagents • Autocomp with cells and CD45+ reagents
BD cells Bead AutoComp BD cells Cell Autocomp
POST SORT REANALYSIS: HUMAN PBL CD3+CD4+ CD45RA+ &CD45RA- Treg+/Teffector+/ -- 98.4 99.4 97.1 99.9
Actual reagents cells compensation Actual reagents beads compensation beads cells cells beads