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H. H. Cheng University of Minnesota 5 October 2008, Houston TX

S-3 Symposium: History of Nitrogen Research: “The Bremner Factor” Early Indicators of the Bremner Factor: Glimpses of Bremner’s Pioneering Research and Their Impact. H. H. Cheng University of Minnesota 5 October 2008, Houston TX. THE BREMNER FACTOR.

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H. H. Cheng University of Minnesota 5 October 2008, Houston TX

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  1. S-3 Symposium: History of Nitrogen Research: “The Bremner Factor”Early Indicators of the Bremner Factor: Glimpses of Bremner’s Pioneering Research and Their Impact H. H. Cheng University of Minnesota 5 October 2008, Houston TX

  2. THE BREMNER FACTOR Agronomy Monograph No. 9: Methods of Soil Analysis (C. A. Black, ed.) 1965. - Chapters on “Total Nitrogen” (p. 1149-1178) - “Inorganic forms of nitrogen” (p. 1179-1237) - “Organic forms of nitrogen” (p. 1238-1255) - “Isotope-ratio analysis of nitrogen in nitrogen-15 tracer investigations” - “Gaseous forms of nitrogen” (p. 1287-1323) - “Nitrogen availability indexes” (p.1324-1345) Agronomy Monograph No. 10: Soil Nitrogen (Bartholomew & Clark, ed.) 1965. - Chapter on “Organic nitrogen in soils” (p. 93-149). Soil Biochemistry Vol. 1 (McLaren & Peterson, ed.) 1967: - Chapter on “Nitrogenous Compounds” (p. 19-66). (341 references)

  3. EARLY PUBLICATIONS OF J. M. BREMNER Bremner et al. 1946. Metallo-organic complexes in soil. Nature 158:770. Bremner, J.M. 1949. Studies on soil organic matter. J. Agr. Sci. 39:183; 274; 280. I . The chemical nature of soil organic nitrogen. II . The extraction of organic matter from soil by neutral reagents.(w/Lees) III. The extraction of organic carbon and nitrogen from soil. 1949-1959: 27 papers (18 single-authored; 9 first-authored) – on methods for organic C, amino acids, amines, amino sugars, inorganic N, and humic acids; on denitrification, fixed ammonium; etc.

  4. Table 85-1. Steam-distillation methods for estimation of different forms of nitrogen in soil hydrolysate (Bremner, 1965. MSA. p. 1244) Forms of N Steam distillation methods Total N with NaOH of Kjeldahl digest Ammonium-N with MgO of acid hydrolysate (Ammonium+ with Phosphate-borate (PB) buffer (pH 11.2) Hexosamine)-N Amino acid-N with PB buffer after removal of ammonium & hexoamine and ninhydrin treatment (pH 2.5) (Ammonium+ with PB buffer after ninhydrin treatment Hexoamine+ Amino acid)-N

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