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DICOM-3 as a standard for virtual slides. Marcial Gª Rojo, Carlos Peces, Javier García marcial@cim.es General Hospital of Ciudad Real Center of Research in Information Technologies of the Castilla-La Mancha Health Service (SESCAM) Spanish Society of Health Informatics Spain.
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DICOM-3 as a standard for virtual slides Marcial Gª Rojo, Carlos Peces, Javier García marcial@cim.es General Hospital of Ciudad RealCenter of Research in Information Technologies of the Castilla-La Mancha Health Service (SESCAM) Spanish Society of Health Informatics Spain
DICOM and Pathology • DICOM WG-13 (Visible Light) • Very large microscope scans of slides problem is assessed in 2003 (Bruce Beckwith, CAP) • DICOM WG-8 (Structured Reporting) • DICOM WG-04 (Compression) • JPEG2000 in DICOM files
Virtual slides project • Background: IHE Pathology Technical Framework in Development • Aim: Integration of pathology images using DICOM • Evaluated systems: • Nikon Coolscope with EclipseNet VSL • Aperio ScanScope T2 • LifeSpan BioSciences Alias II • Olympus/Soft Imaging System .slide • Bacus BLISS (CNIO, Spain) • Zeiss Mirax Scan (Demo presentation)
File size and different formats • PACS systems manage images which are DICOM-compliant. • DICOM files are limited in size to 2GB. • Most DICOM systems cannot manage TIFF files with a tiled organization, and/or TIFF files with JPEG or JPEG2000 compression. • For these reasons it is generally not possible to import proprietary files (Aperio .SVS, Olympus-SIS .VSI,…) directly into PACS.
…Image transmission • PACS systems use a store-and-forward idiom. All image data are stored on a central server. • The entire image is transmitted from the server to the client, and loaded into the client’s memory. • Large images are slow to load, slow to view, and slow to process. • Images larger than about 500MB are impossible to process.
Solution: single JPEG2000 as DICOM object • JPEG2000 gives excellent compression ratios • Proprietary files (SVS) are used for initial pathology diagnosis. • When cases are signed, they are converted into JPEG2000. • PACS system is able to manage JPEG2000 files (<2GB) • JPEG2000 large single file is embedded as DICOM object (PixelMed DICOM toolkit, modified) PACS • When requested from PACS, JPEG2000 files are fragmented into small chunks (Kakadu-based algorithm, high computing resources) Common image viewer • If proprietary (SVS, VSI,..) files are needed later on (by Pathology Dept.) , an algorithm is used to reconstruct those proprietary files.
Schema Pathology Dept Image processing intermediary server Other medical departments (web viewer)
Solutions for web viewer • Cutting large images into small tiles: • Small JPEG pieces. Java application. A similar method is used by ZoomifyER • JPEG2000 viewer. Java application based in Kakadu library
Our Proposal for DICOM • SESCAM CITI DICOM Conformance Statement available on request: cpeces@sescam.jccm.es • Field Value • Transfer syntax JPEG2000 lossless • SOP Class VL Microscopic Image Storage • Modality GM (General Microscopic) • Photometric interpretation YBR_RCT (instead of RGB, to use JPEG2000 reversible color transform)
Transmission between PACS and web viewer • DICOM transference (C-MOVE o C-GET/C-STORE) • Standard protocols (JPEG/HTTP, J2K/JPIP) • Proprietary protocol • DICOM Persistent Object transference (WADO/HTTP). Our next step