Catalysis: Concepts and Green Applications

1. Catalysis: Concepts and Green Applications Lecture slides for Chapter 5: Biocatalysis Most of the graphics here were drawn using PowerPoint and Chemdraw (version Ultra 9.0). Feel free to modify and/or add your own pyrotechnics. Please send any feedback to feedback@catalysisbook.org

2. Bulk chemicals Food Fine chemicals Detergents Biocatalysis applications Pharmaceuticals Fibres Nutraceuticals Animal feed Bioremediation Pulp and paper Figure 5.1

3. Figure 5.2

4. Figure 5.3

5. Figure 5.4

6. Figure 5.5

7. Figure 5.6

8. Figure 5.7 colour fig

9. fast slow enzyme-bound acyl intermediate fast Figure 5.8

10. enzyme I enzyme substrate-H2 substrate substrate substrate-H2 NAD(P)H NAD(P)+ NAD(P)H NAD(P)+ second substrate-H2 second substrate second substrate-H2 second substrate enzyme II Figure 5.9

11. + enzyme Eupergit resin Figure 5.10

12. Figure 5.11

13. cross-linking precipitation enzyme molecules ~5 nm aggregates ~1 mm CLEAs, 1–100mm Figure 5.12

14. pathway blocked isatin hydrolase naphthalene dioxygenase (NDO) Figure 5.13

15. penicillin acylase H2O, 37°C penicillin G 6-aminopenicillanic acid (6-APA) CH3SiCl H2O – 40°C PCl5 n-BuOH ampicillin (R = H), amoxycillin (R = OH) cephalexine Figure 5.14

16. 8-aza-1-tetralone Figure 5.15

17. Figure 5.16

18. Figure 5.17

19. amino acid sequence select improved sequence mutated sequences selection cloning screening Figure 5.18

20. antigens (haptens) antigen binding site light chain S-S bond hinge heavy chain Figure 5.19

21. Figure 5.20

22. Figure 5.21

23. D-glucose pretreatment isoglucose (42% D-fructose) concentration HFCS (45% D-fructose) cooling filtration on charcoal reactor series, 55–65 °C Figure 5.22

24. Figure 5.23

25. Figure 5.24

26. 11 chemical steps 13 reagents 13 solvents 10-diacetylbaccatin (10-DAB) isolation and crystallising plant cell fermentation in aqueous solution paclitaxel Figure 5.25

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28. Figure 5.27

29. Figure 5.28

30. Figure 5.29