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Objectives

Prokaryotic Gene Regulation April 16, 2014 Richard D. Howells, PhD MSB E-643 howells@njms.rutgers.edu. Objectives. During this presentation, you may Continue catching up on needed sleep Understand the process of transcription

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Objectives

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  1. Prokaryotic Gene Regulation April 16, 2014Richard D. Howells, PhDMSB E-643howells@njms.rutgers.edu

  2. Objectives During this presentation, you may • Continue catching up on needed sleep • Understand the process of transcription • Appreciate how the lac and trp operons are regulated in bacteria Lecture 13

  3. General structure and tandem arrays of pre-rRNA transcription units

  4. Transcription nomenclature

  5. The bacterial RNA polymerase

  6. Bacterial RNA Polymerase

  7. Inactivation of prokaryotic RNA polymerase by Rifampin - a drug used for treatment of tuberculosis

  8. Prokaryotic promoter region

  9. The Lac Operon

  10. Lactose Metabolismin E. coli

  11. Transcriptional Regulation of the Lac Operon

  12. The Lac Operon Promoter

  13. The lac promoter vs. the promoter consensus sequence

  14. Structure of cAMP

  15. Control of lacoperon byglucose and cAMP

  16. cAMP-CAP (CRP) homodimer (yellow domain interacts with RNA pol)

  17. tTTheT The cAMP-CAP complex activates transcription of the lacoperon

  18. Lac Operon Regulation

  19. Regulation of the trp operon in E. coli

  20. Trp repressor homodimer bound to Trp and the trp operator

  21. Rho-independent termination of transcription

  22. Transcriptional attenuation in the trp operon

  23. Transcriptional attenuation in the trp operon

  24. Transcriptional attenuation in the trp operon

  25. Attenuation in the trp operon

  26. Leader sequences of other AA operons in E. coli subject to attenuation

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