SCENAR-therapy as a universal method for managing oxidative stress

1. SCENAR-therapy as a universal method for managing oxidative stress Emergency Care Department, Rostov State Medical University, Rostov-on-Don, RITM OKB ZAO, Taganrog, Russia L.V. Klimova, A.V. Tarakanov, A.J. Cherchago

2. Oxidative stress Increases the deleteriouseffect of: xenobiotics, hypoxia, ischemiaandreperfusion, inflammation, bacterialand virus infections, alcohol, hyperglycemia(diabetes), ... Greatly influences on: reproduction, ontogenesis and cell proliferation, carcinogenesis, atherogenesis, ageingprocesses, …

3. Oxidative stress isprooxidant – antioxidant disbalance with prooxidants prevailing and antioxidant systems astrain

4. What is prooxidant?

5. Oxygen Makes 65% of the human body weight We breath in400 litres ofО2 daily “The amount of free oxygen is the chemical measure for the living matter” V.I. Vernadsky

6. Physiological norm 95-98%of the used О2goes on producing energy and providing oxidative catabolism 2-5%turns into prooxidantsthat in physiological conditions play the key role in the cell metabolism, maintain the homeostasisof the body internal environmentand its adaptive reactions.

7. Prooxidantsare: activated oxygen metabolites, active oxygen forms, free radicals of this etiology

8. In pathology the amount of prooxidants increases and free radical oxidation activation processes of all boimolecules, in particular lipids of biological membranes, go out of control.

9. Structure of the cell membrane

10. Lipid peroxidation products produce toxic effect due to biopolymer linking, mitochondrialswelling and divisionof oxidativephosphorylation, as well as inactivation of enzymes that are involved in breathing and glycolysis. They oxidate proteins, damage DNA, can slow down and even stop division and growth of cells, destabilize cell membranes.

11. ANTIOXIDANT is any substance which, when present at low concentrations relative to the substrate being oxidized, will inhibit or retard that oxidation Halliwell B., Gutteridge J.M.C. (1989)

12. Antioxidants Differ in chemical nature, dissolubility, molecularmass, cell specificity, action mechanism and principle. Antioxidants are enzymes, polyphenols, flavonoids, hormones, vitamines, selenic and thioiccompounds, carnosine, ubiquinones, etc. They are regenerated in the body non-stop, as well as come with food or antioxidative drugs.

13. Antioxidants canbeeitherdirectorindirectintheireffect

14. Direct antioxidants act in vivo, as well asin vitro. Direct antioxidants are most endogenic and synthetic antioxidants. Commonly antioxidants are functionally connected with each other, can be in synergetic or antagonistic interaction, have a dose-dependent effect, and thus the drug-management of the oxidative stress in any pathology can be problematic.

15. Indirect antioxidants act onlyin vivo, i.e. in the alive systems by stimulating the synthesis of endogenic antioxidants, stabilization of cell membranes and normalization of the metabolic processes. Such an effect can be when using not only chemical substances, but a number of non-drug therapies (for example, SCENAR-therapy).

16. Since late 90th the influence of SCENAR-therapy on the processes associated with oxidative stress in patients with various acute and chronic pathologies is in the focus of the researches. Most biochemical researches were performed in biochemical laboratory (Research Institute of Biology, Southern Federal University) by researches from SFU and Rostov State Medical University, as well as doctors from several medical institutions of Rostov-on-Don performed laboratory researches. Some research activities were performed in the Perm Medical Academy.

17. Pilot Scientific Research

18. Acute experimentalpancreatitis(2006) Experimental animals– lab rats with various stages of acute experimental pancreatitis Research organisation: Rostov State Medical University To administer SCENAR-therapy researches have constructed special cell in the body of the lab animals which will enable stimulation with SCENAR 97.4 of the inner side of the rat’s paw setting the stimulation energy that doesn’t involve painful sensations at a 60Hz frequency. Treatment course: 10 sessions, 15 minutes each, daily, beginning with the first AEP. Blood plasma and erythrocytes were analyzed

19. HBO-inducedoxidativestress (2009) Experimental animals - lab white rats that were exposed to hyperbaric oxygenation (HBO) Research basis: Department of Biochemistry and Microbiology, Southern Federal University, Rostov-on-Don In the research the experimental group of rats after the HBO-exposure (1 hour at a pressure of 0.5 MPa in the special pressure chamber with the alkaline absorber) underwent 5 daily SCENAR-sessions, 5 minute each. Blood plasma,erythrocytes, brain and liver of the animals were analyzed.

20. Clinical Scientific Research

21. Expirimental groups – patients underwent multiple therapy, including SCENAR-therapy Control groups – patients underwent only various common therapies

22. SCENAR-therapy: General Treatment Techniques 3 pathways and 6 points and collar zone, forehead, adrenal glands Treatment techniques were altered Stimulation energy – comfortable

23. 3 pathways, 6 points Subjectively dosed mode with the labile-stabile stimulation technique

24. 3 pathways, 6 points Individual dosed mode with the stabilestimulation technique based on automatic measurement of skin reactions change and evaluation of these changes

25. Collar zone, forehead, adrenal glands skin projection Stimulation scheme and method procedure for ‘collar zone, forehead, adrenal glands’in the SDM with the labile-stabile stimulation

26. Collar zone, forehead, adrenal glands skin projection Individual dosed mode with the stabile stimulation based on automatic measurement of skin reactions change and evaluation of these changes

27. Research methods and tests Plasma: • chemiluminescence analysis • lipid peroxidation products content • total peroxidaseactivity • catalase and ceruloplasminoxidaseactivity • amount of ectoglobularhemoglobin • nitrozilhemoglobincontent Erythrocytes: • content of lipid peroxidation products • superoxidedismutase and catalase activity • structuralindices of erythrocytemembranes

28. Earlypostinfarctionangina(2003) Experimental group (SCENAR-therapy) – 20 patients Control group– 20 patients Medical Institution : Rostov Regional Cardiological Sanitarium Complementary part of SCENAR-therapy : stimulation of the neck and chest anterior surface, inner side of the arm and heart area. The stimulation of the abdominal wall was complemented or altered in the treatment (solar plexus area, lower abdomen). Treatment course: 10 sessions with 2-3 day break

29. Relapse of duodenalulcer(2004-2005) Experimental group (SCENAR-therapy) – 36patients (monotherapy), 50 patients (multiple therapy) Control group– 25patients Medical Institution : Perm State Medical Academy Complementary part of SCENAR-therapy : stimulation of the epigastrium area following the reflexosegmental technique. Treatment course: 10 daily sessions, 30 minutes each

30. Acute renal failure caused by compression injury (2005) Experimental group (SCENAR-therapy) – 25patients Control group– 41patients Medical Institution : Department of Acute Hemodialysis, City Emergency Hospital №2, Rostov-on-Don. The treatment course started on patient’s admissionand in average included 8-10 daily sessions, was finished as the diuresis recovered.

31. Q-wave AMI(2007) Experimental group (SCENAR-therapy)– 30 patients Control group– 47patients Medical Institution : Cardiological Department, City Hospital №2, Rostov-on-Don Complementary part of SCENAR-therapy: stimulation of the pericardium meridian (МС4, МС6 and МС7) on the left and right arm for 2-3 minutes, as well as stimulation of the complaint Here and Now – treatment of the pain projections, if any. Treatment course: 10-14 sessions, every day

32. Chronic insomnia (2007) Experimental group (SCENAR-therapy)– 20 patients Control group– 53 patients Patients: emergency doctors from different hospitals of Rostov-on-Don and Rostov region. Complementary part of SCENAR-therapy: stimulation of the hypersensitive zones, areas of muscle hyper- and hypertension in the zones connected with the pathological focus and out of them. Treatment course: 10 sessions, every other day

33. Ischemicheartdisease(2009) Experimental group (SCENAR-therapy) – 50 patients Control group– 73 patients Medical Institution : Cardiological Department, City Hospital №7, Rostov-on-Don SCENAR-therapy features: one general technique ‘3 pathways, 6 points’ in the IDM, 60Hz frequency. Treatment course:10-day course, morning session every day after breakfast

34. SCENAR-therapy:local techniques stimulation with the add-on probes (12 сm2) in the F-Swmode of skin zones in the hand area (thenarandhypo thenar) and feet (space under toes ofregio plantaris pedis) withfinalstimulation of the liver projection (including F13 andF14 points and liver meridian), 10 min for each zone. Stimulation energy was selected individually in each particular case.

35. Appendicularpurulentperitonitis(2008) Experimental group (SCENAR-therapy) – 38 patients Control group– 42 patients Medical Institution: Department of Purulent Surgery, City Emergency Hospital №2, Rostov-on-Don Treatment course: 5-day course, daily morning sessions

36. Finding 1 Including SCENAR-therapy into the multiple therapy of acute and chronic diseases contributes to management of oxidative stress in the blood plasma and erythrocytes by inhibiting prooxidant production and high activity of lipid perioxidation and regulation of the activity of body antioxidant system state

37. Finding 2 Antioxidative effect is achieved by treating following both general and local SCENAR-techniques

38. Finding 3 Indirect antioxidative effect of SCENAR-THERAPY prevails over antioxidants of direct effect as it normalizes the balance between the oxidative and antioxidative processes by activating natural universal processes of sanogenesis

39. Finding 4 Inhibition the oxidative stress is one of the signs of evident positive clinical effect of SCENAR-therapy

40. Thank You