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Impacts of Hypoxia on Reproductive Function in the Gulf Killifish, Fundulus grandis

Impacts of Hypoxia on Reproductive Function in the Gulf Killifish, Fundulus grandis. Ann Oliver Cheek. juvenile. Blue crabs. Atlantic croaker. 3 ° consumers. juvenile. larval. Gulf killifish. 2 ° consumers. 1 ° consumers. Grass shrimp. Benthic consumers. Chl a. 1 ° producers.

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Impacts of Hypoxia on Reproductive Function in the Gulf Killifish, Fundulus grandis

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  1. Impacts of Hypoxia on Reproductive Function in the Gulf Killifish, Fundulus grandis Ann Oliver Cheek

  2. juvenile Blue crabs Atlantic croaker 3° consumers juvenile larval Gulf killifish 2° consumers 1° consumers Grass shrimp Benthic consumers Chl a 1° producers N, P nutrients decomposition N nutrients Land Use N cycle Bacteria Aerobic Bacteria

  3. GOALS & HYPOTHESIS • Develop indicators of hypoxic effects in a common marsh resident – Fundulus grandis • Advance understanding of how hypoxia regulates reproduction in fish • Hypoxia impairs reproduction

  4. Gulf killifish

  5. REPRODUCTIVE LIFE HISTORY Batch Spawner Reproductive Parameters J F M A M J J A S O N D

  6. INDICATORS of EFFECT • Fulton’s condition factor (100,000*W/L3) • Gonadosomatic index (100*Wgonad/Wbody) • Estradiol (females) • Testosterone (males & females) • 11-Ketotestosterone (males) • Vitellogenin (females)

  7. VITELLOGENIN FSH Reproductive Hormone Function brain liver pituitary ovary estrogen testosterone

  8. T, 11KT FSH, LH Reproductive Hormone Function brain liver pituitary testis

  9. Laboratory ExposureChronic Hypoxia 15 ppt salinity 27°C

  10. Chronic HypoxiaFemales

  11. Chronic HypoxiaEgg Production

  12. Chronic HypoxiaMales

  13. How do indicators respond in GOM estuaries? Pensacola Bay, FL Weeks Bay, AL

  14. Weeks Bay Mouth Weeks Bay Creek Mobile Bay

  15. 2 mg/L  41 mmHg @ 31C

  16. Severe Hypoxia reduces Sex Steroid Concentrations WBS = Mild hypoxia WBC = Severe hypoxia, PO2 ~ 82 mmHg PO2 ~ 19 mmHg ***p < 0.001

  17. 2 mg/L  41 mmHg

  18. Moderate Hypoxia reduces GSI & 11KT WBS = Normoxic, PO2 ~ 91 mmHg WBC = Hypoxic, PO2 ~ 51 mmHg **p < 0.01, ***P < 0.001

  19. Indian Bayou Garcon Point

  20. 2 mg/L  41 mmHg

  21. Moderate Hypoxia reduces GSI & 11KT GP = Normoxic, PO2 ~ 89 mmHg IB = Hypoxic, PO2 ~ 49 mmHg **p < 0.01, ***P < 0.001

  22. REPRODUCTIVE IMPACT Not changed by hypoxia Reduced by hypoxia

  23. Reproductive Impacts of Diel Hypoxia • In the field • Male steroid hormones & GSI are reduced • Female GSI can be reduced • In the lab • Male steroid hormones are reduced • Female steroid hormones & GSI are reduced • Fecundity is reduced

  24. Acknowledgments Christie Landry, Amy Sutton, Stacy Steele, Brett Henry, Sarah Temple, Lisa Cohen, Bern Wood, Ross Turlington; SLU Dick Snyder & lab; UWF Steve Manning, Thea Hoexum-Brouwer; USM Scott Phipps, Eric Brunden; Weeks Bay NERR Shaye Sable, LSU Chad Ellington, UNO

  25. Cyclic Hypoxia

  26. Cyclic Hypoxia Steroids & GSI Males Females

  27. 50 40 30 20 10 0 8/3/06 8/10/06 8/17/06 8/24/06 8/31/06 Cyclic Hypoxia Egg Production 6.48 mg/L 1.4 mg/L Daily Eggs/Female

  28. Cyclic Hypoxia Fertilization

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