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Intrathecal Drug Targeting: Magnetically Guided Nanoparticles

Intrathecal Drug Targeting: Magnetically Guided Nanoparticles. Tejen Soni Professor Andreas Linninger Eric Lueshen Indu Venugopal. Goals. Build a spinal cord model with stent Measure nanoparticle retention Time Field Strength

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Intrathecal Drug Targeting: Magnetically Guided Nanoparticles

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  1. Intrathecal Drug Targeting:Magnetically Guided Nanoparticles Tejen Soni Professor Andreas Linninger Eric Lueshen Indu Venugopal

  2. Goals • Build a spinal cord model with stent • Measure nanoparticle retention • Time • Field Strength • Compare collection efficiency against previous stent-less model • Run simultaneous stentlessmodel experiments • Synthesize magnetic nanoparticles • Later on: • Perform the same experiments for a vasculature model • Develop a more accurate/realistic model

  3. Stent • 8mm diameter • Stainless steel stent • Nitinol stents are not magnetic • Increases magnetic strength • Strength of magnetic field does not come from size of magnet • Field strength is increased by the gradient of the magnetic field • F = grad(m ∙ B)

  4. Spinal Cord Model • 1 stainless steel stent • 2 falcon tubes • 2 PVC pipe valves • 3 pipette valves • 44 nerve roots • 35 lb pull force target magnet • 130 lb pull force barrier magnet • Systolic pump • Syringe pump

  5. Experiments • Systolic pump mimics pulsating motion of CSF • Unlike vascular system where blood flows • Systolic pump: 72 beats/min • Syringe pump: 0.5 ml/min for 2 minutes Lower Upper 2 3 1 Magnetic Barrier Targeting Magnet

  6. Retention vs. Time • Input nanoparticles at valve 1 • Measure collection efficiency in each zone after 1, 5, and 10 minutes • Running each test with fixed target magnet strength • Plot and compare data to the stent-less model Lower Upper 2 3 1 Magnetic Barrier Targeting Magnet

  7. Retention vs. Field Strength • Input nanoparticles at valve 1 • Measure collection efficiency in each zone for 9.5, 25, and 35 lb pull force targeting magnets • Running each test for a fixed amount of time • Plot and compare data to the stent-less model Lower Upper 2 3 1 Magnetic Barrier Targeting Magnet

  8. Stent-less Model Results

  9. Stent-less Model Results

  10. Synthesizing Nanoparticles • 50 ml of 0.5 M ammonium hydroxide to 5 ml iron chloride solution • Black precipitate forms • Magnetic nanoparticles • Centrifuge and decant supernatant • Mix with pure water and store

  11. Future Work • Develop a more accurate/realistic model • Stent suspended in canal • Stent around the dura (falcon tubes) • Run repeated stentless model experiments • Perform the same experiments for a vasculature model • Using soft tube as an artery

  12. References • HenrikKempe, Maria Kempe, The use of magnetite nanoparticles for implant-assisted magnetic drug targeting in thrombolytic therapy, Biomaterials, Volume 31, Issue 36, December 2010, Pages 9499- 9510, ISSN 0142-9612, 10.1016/j.biomaterials.2010.07.107. (http://www.sciencedirect.com/science/article/pii/S0142 96121001001X) • Mangual, Jan O., Shigeng Li, Harry J. Ploehn, Armin D. Ebner, and James A. Ritter. "Biodegradable Nanocomposite Magnetite Stent for Implant-assisted Magnetic Drug Targeting." Journal of Magnetism and Magnetic Materials 322.20 (2010): 3094-100. Elsevier. Web. • Linninger, Andreas, Eric Lueshen, and MadhawaHettiarachchi. "Magnetically Guided Nanoparticles for Targeted Drug Delivery into the Brain and Central Nervous System." Laboratory for Product and Process Design, LPPD Department of Bioengineering, University of Illinois at Chicago. Web. <http://vienna.bioengr.uic.edu/research/MNP_Drug_Delivery.pdf>.

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