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Control siRNA

A. B. c-MYC siRNA. c-MYC siRNA. c-MYC siRNA. Control siRNA. Control siRNA. Control siRNA. c-MYC siRNA. Control siRNA. 250. 200. 150. p21 protein expression (% control). 100. 50. 0. p21 (21 kDa). β -Actin (42 kDa). D341. D425. DAOY wt. DAOY M2. DAOY V11. DAOY wt. 1. 0.8.

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Control siRNA

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  1. A B c-MYC siRNA c-MYC siRNA c-MYC siRNA Control siRNA Control siRNA Control siRNA c-MYC siRNA Control siRNA 250 200 150 p21 protein expression (% control) 100 50 0 p21 (21 kDa) β-Actin (42 kDa) D341 D425 DAOY wt DAOY M2 DAOY V11 DAOY wt 1 0.8 Proliferative activity (OD 490nm) 0.6 0.4 120 0.2 0 Day 0 Day 2 Day 4 Day 6 100 * * DAOY V11 D341 80 *** 1 1 *** Clonogenic survival (% control) 60 0.8 0.8 Proliferative activity (OD 490nm) Proliferative activity (OD 490nm) * 0.6 0.6 40 0.4 0.4 0.2 0.2 20 0 0 Day 0 Day 2 Day 4 Day 6 Day 0 Day 2 Day 4 Day 6 0 D425 DAOY M2 1 1 D341 D425 0.8 0.8 DAOY wt DAOY M2 Proliferative activity (OD 490nm) Proliferative activity (OD 490nm) DAOY V11 0.6 0.6 0.4 0.4 0.2 0.2 0 0 Day 0 Day 2 Day 4 Day 6 Day 0 Day 2 Day 4 Day 6 C D 80 DAOY wt * 60 Total Cell count in % 40 20 0 G1 S G2/M *** ** 80 80 DAOY V11 D341 60 60 Total Cell count in % Total Cell count in % ** 40 40 20 20 0 0 G1 S G2/M G1 S G2/M * 80 80 D425 DAOY M2 60 60 * Total Cell count in % Total Cell count in % 40 40 20 20 0 0 G1 S G2/M G1 S G2/M

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