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Control siRNA

Selected siRNA. Control siRNA. A. B. CTSS. CTSS. Selected siRNA. Control siRNA. 1 0.2 . CD137L. CD137L. 1 0.1 . EFNA3. MDK. 1 0.2. 1 0.2 . GDF15. NEO1. 1 0.2 . 1 0.1 . GTF2I. SERPINI1. 1 0.5 . 1 0.2 . MDK. PODXL.

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Control siRNA

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  1. SelectedsiRNA Control siRNA A B CTSS CTSS SelectedsiRNA Control siRNA 1 0.2 CD137L CD137L 1 0.1 EFNA3 MDK 1 0.2 1 0.2 GDF15 NEO1 1 0.2 1 0.1 GTF2I SERPINI1 1 0.5 1 0.2 MDK PODXL 1 0.1 1 0.3 NEO1 SOSTDC1 1 0.2 1 0.2 SERPINI1 Tubulin 1 0.1 1 0.1 PODXL 1 0.1 1 0.1 SOSTDC1 1 0.1 ZG16B 1 0.1 GAPDH Supplementary Figure S5. Knock-down confirmation by PCR and western blot of the indicated siRNAs targets. A, Representative semi-quantitative PCR images showing the down-regulation at mRNA level of the indicated siRNAs targets. GADPH was used as internal control of the assay. B, Representative western blotting analyses showing the effectiveness of the knock-down at protein level of targets with available antibodies. Tubulin was used as internal control of the assay.

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