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The wonderful duplicating “machine”

The wonderful duplicating “machine”. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.)

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The wonderful duplicating “machine”

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  1. The wonderful duplicating “machine”

  2. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach) A briefe history of the cell cycle

  3. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  4. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  5. ONCE and ONLY ONCE

  6. Fluorescent Activated Cell Scan/Sorter (FACS)

  7. A typical FACS analysis

  8. Methods for cell synchronisation

  9. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase () 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  10. G1 M M Cell fusion experimentJohnson and Rao 1971 S M M G2 M M

  11. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  12. Maturation promoting factor cytostatic factor

  13. Discovery of MPFMasui and Markert 1971

  14. Oscillations of MPF

  15. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  16. Model organisms I - Budding yeast (S.cerevisiae) L. Hartwell

  17. Model organisms II - Fission yeast (S.pombe) P. Nurse

  18. Generation of temperature sensitive budding yeast mutants

  19. Generation of temperature sensitive budding yeast mutants

  20. Analysis of ts cell cycle mutants S M M M G1 M

  21. Identification of cell cycle ts mutants

  22. Some ts mutants which became famous

  23. The two models of cell cycle regulation

  24. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  25. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) 1983 Discovery of the first cyclins (Hunt) 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)

  26. Oscillations of mitotic cyclins are required for MPF activity

  27. MPF = cdc2 + cyclin B

  28. Regulation of mitotic cyclin levels in cycling cells

  29. Cdc2/28 (=cdk1) is regulated by: 1. Binding of a cyclin2. Inhibitory phosphorylation3. Activating phosophorylation4. Binding of CKI5. Cellular localization

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