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新衍生的食品中毒細菌 ENTEROTOXIGENIC BACTEROIDES FRAGILIS (ETBF)

新衍生的食品中毒細菌 ENTEROTOXIGENIC BACTEROIDES FRAGILIS (ETBF). 特性. 格蘭式陰性短桿菌 不產孢子 厭氧 毒素是 Bacteroides fragilis toxin(BFT) ( 一種 extracellular heat-labile protein toxin) 作用在細胞骨架，使腸道表皮細胞變形. Bacteroides fragilis toxin(BFT). 1983 年在羊群腹瀉的糞便中發現 , 刺激羊的結腸的分泌反應 1987 年在急性腹瀉成人和小孩的糞便中發現 依據能否分泌 BFT 來將菌種分類為

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新衍生的食品中毒細菌 ENTEROTOXIGENIC BACTEROIDES FRAGILIS (ETBF)

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  1. 新衍生的食品中毒細菌ENTEROTOXIGENIC BACTEROIDES FRAGILIS(ETBF)

  2. 特性 • 格蘭式陰性短桿菌 • 不產孢子 • 厭氧 • 毒素是Bacteroides fragilis toxin(BFT) (一種extracellular heat-labile protein toxin) • 作用在細胞骨架，使腸道表皮細胞變形

  3. Bacteroides fragilis toxin(BFT) • 1983年在羊群腹瀉的糞便中發現,刺激羊的結腸的分泌反應 • 1987年在急性腹瀉成人和小孩的糞便中發現 • 依據能否分泌BFT來將菌種分類為 enterotoxigenic Bacteroides fragilis(ETBF) nonenterotoxigenic Bacteroides fragilis(NTBF) • 有3種BFT異構體,即BFT-1 ,BFT-2 ,BFT-3

  4. 致病性 • 除了家畜外,人類也會感染 • 成人大多感染的是bft-1 • 兒童大多感染的是bft-2(其中1-5歲幼兒腹瀉感染關係較為密切) • 也可能致癌 • 致病機制:改變纖維肌動蛋白的結構,裂解黏著小蛋白(E-鈣黏素),導致腸上皮細胞的緊密聯繫被破壞增加了通透性

  5. 鑑定&耐受性 • 用 primers 404/407 和PCR 去鑑定B. fragilis enterotoxin (fragilysin) 基因 • 菌株對抗生素的耐受性由藥物敏感性測試(Etest)決定 (對amoxicillin/clavulanic acid, imipenem and metronidazole是 susceptible ) • 在體外(in vivo)實驗中, 和 beta-lactamase inhibitors, carbapenems and metronidazole結合的beta-lactams 最有效用

  6. Gram stain of Enterotoxigenic B. fragilis (ETBF) under oil immersion. 100x.

  7. Tumors in the colons of multiple intestinal neoplasia (Min) mice colonized with enterotoxigenic Bacteroides fragilis (ETBF) for 3 months compared to Min mice not colonized with ETBF. The colons are stained with methylene blue.

  8. References： • CAT.INIST Cat.inist or about 15 million bibliographic records (back to 1973) of documents held in the INIST/CNRS collections and covering all fields of worldwide research in Science, Technology, Medicine, Humanities and Social Sciences. • http://cat.inist.fr/?aModele=afficheN&cpsidt=3582450 • Myers LL, Shoop DS, Stackhouse LL, Newman FS, Flaherty RJ, Letson GW, Sack RB. Isolation of enterotoxigenic Bacteroides fragilis from humans with diarrhea.J Clin Microbiol. 1987 Dec;25(12):2330-3. • Vu Nguyen T, Le Van P, Le Huy C, Weintraub A.Diarrhea caused by enterotoxigenic Bacteroides fragilis in children less than 5 years of age in Hanoi, Vietnam.Anaerobe. 2005 Feb-Apr;11(1-2):109-14. Epub 2004 Dec 9. • http://www.blackwellpublishing.com/eccmid14/abstract.asp?id=14528 • http://www.cqvip.com/onlineread/onlineread.asp?ID=15949818&SUID=EGBNBFDHDNCBCOPIBOLGEMCBCDOIPNFP#

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