1 / 7

Enzyme Catalysis with Catalase

Enzyme Catalysis with Catalase. Key Notes From Lab Handout. Enzyme: Catalase (from beef liver) Substrate: H 2 O 2 (hydrogen peroxide) Stop Reaction: use sulfuric acid (H 2 SO 4 ) Quantify Amt. of H 2 O 2 left: titrate in KMnO 4 (potassium permanganate)

mary
Download Presentation

Enzyme Catalysis with Catalase

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Enzyme Catalysis with Catalase

  2. Key Notes From Lab Handout • Enzyme: Catalase (from beef liver) • Substrate: H2O2 (hydrogen peroxide) • Stop Reaction: use sulfuric acid (H2SO4) • Quantify Amt. of H2O2 left: titrate in KMnO4 (potassium permanganate) • Baseline: Starting total amount of H2O2 • FOUR ways enzyme activity can be altered: • Salt concentration (salinity) • pH (acid/base concentration) • Temperature • Activators & Inhibitors

  3. Lab #3- Enzyme Catalysis w/Catalase Three Parts to the lab: • Establish Baseline Amount of H2O2 • Uncatalyzed Decomposition of H2O2 • Time Trials w/Catalase to determine Rxn rate • Procedure: • 10 ml H2O2 in a beaker • 1.0 ml (H2O or Catalase) • 10 ml 1 M H2SO4 • Mix well • Take a 5 ml sample and titrate in KMnO4 • Read Initial and final measurements on buret • Record Data

  4. Sample Data Set I

  5. Sample Data II

  6. Conclusion Questions to Consider • Determine the amount of H2O2 in moles using the chemical equation on pg. 22 and your amount of H2O2 after 180 sec. • From Question #6, Choose two factors that can affect the rate of enzyme-catalyzed reaction of Catalase. Draw a predicted graph for each reaction. • Name two organisms that could be used to determine the reaction rate of an enzyme. • List two major sources of error and one suggestion on how to improve the lab. What would you do differently?

  7. You can use either Set I or Set II if your data did not come out right!!! When doing your analysis be sure to use good data especially if your values did not come out similar to what set I & II show!!!

More Related