objectives

1. objectives To differentiate between the two major categories of bacteria: Gram positive and Gram negative. To understand how the Gram stain reaction affects Gram positive and Gram negative bacteria based on the biochemical and structural differences of their cell walls. To identify different structures of bacteria.

2. Importance of a Gram Stain: Classification Diagnosis (direct smear) Antimicrobial selection (treatment) Statistical analysis and Epidemiology

6. Gram stain technique

7. Gram negativr Rod (E coli)

9. Streptococcus pyogenes

31. Spore stain

32. Bipolar stain

33. Mycobacterium tuberculosis

34. Capsule stain

35. Mycobacterium leprae

36. Gram positive cocci

38. Vibrio cholerae on TCBS

39. Klebsiella species on CLED

40. Blood Agar

41. Chocolate Agar

42. Eosin Methylen Blue Agar

43. Eosin Methylen Blue Agar

44. Mac Conkey Agar

45. Mannitol Salt Agar

46. Tissue Culture

47. Inoculation of Embryonated egg

48. Embryonated egg(Inoculation Sites)

49. Citrate Utilization Test

50. Urease Test