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Microtubules and polarized growth in Aspergillus nidulans

Microtubules and polarized growth in Aspergillus nidulans. Reinhard Fischer Max-Planck-Institute for Terrestrial Microbiology. Myosin. Actin. MT. Polarised growth and the Spitzenkörper. B. Robertson, Athens GA. Conventional kinesin is required for

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Microtubules and polarized growth in Aspergillus nidulans

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  1. Microtubules and polarized growth in Aspergillus nidulans Reinhard Fischer Max-Planck-Institute for Terrestrial Microbiology

  2. Myosin Actin MT Polarised growth and the Spitzenkörper B. Robertson, Athens GA

  3. Conventional kinesin is required for hyphal extension (Natalia Requena) Wild type DkinA Requena et al., 2001, Mol. Mirobiol. 42: 121-132

  4. Myosin Actin MT Polarised growth and the Spitzenkörper Conventional Kinesin B. Robertson, Athens GA

  5. Maekawa et al., 2003, EMBO J. 22: 438-449 Cortical interactions of microtubules: Nuclear migration in S. cerevisiae Num1 (ApsA) Yamamoto et al., 2003, JCS 116:4501-4512

  6. Polarity mutants in S. pombe Dtea1 and Dtea2 Browning et al., 2000, JCB, 151:15-27

  7. Microtubule organization inDtea1 and Dtea2 strains WT Dtea1 Dtea2 Browning et al., 2000, JCB, 151:15-27 Mata and Nurse, 1997, Cell 89:939-949 Tea1 destabilizes microtubules Tea2 stabilizes microtubules

  8. KipA deletion affects polarized growth (Sven Konzack) Wild type kipA (tea2) mutant

  9. wild type kipA-deletion KipA deletion affects Spitzenkörper position

  10. wild type DkipA KipA is required for microtuble organization in the tip Difference to S. pombeDtea2

  11. GFP GFP KipA localization Sven Konzack

  12. mRFP1-Microtubules GFP-KipA Microtubule dynamics Xin Xiang, Ron Morris KipA is delivered in the tip Morris, Curr. Opin. Cell Biol. 15:54-59, 2003 Efimov, Mol. Biol. Cell 14:871-888, 2003 Zhang et al., Mol. Biol. Cell 15: , 2003

  13. KipA KipA KipA KipA Microtubule plus end TeaA? Hestermann et al., 2002

  14. KipA and polarized growth DkipA Wild type KipA TeaA? Conventional Kinesin Putative TeaA receptor

  15. wild type DkipA DteaA DkipA and DteaA display distinct phenotypes

  16. AlpA? KipA KipA KipA KipA Microtubule plus end Does KipA transport other cargoes? Hestermann et al., 2002

  17. GFP-MT mRFP1-AlpA 0 2:27 Localization of AlpA in A. nidulans(Cathrin Enke) Mitosis GFP-AlpA

  18. WT DalpA Curved hyphae 25°C 37°C 42°C Deletion of alpA

  19. Microtubule plus end Curved hyphae KipA AlpA (XMAP) NudF (LIS1) Cortical protein: TeaA No curved hyphae NudA (dynein) Cortical protein: ApsA Hestermann et al., 2002

  20. ApsA TeaA? AlpA NudF NudA KipA KinA Different cortical interactions for nuclear migration and polarized growth

  21. mRFP-Microtubules GFP-KipA Microtubule polarity in A. nidulans Microtubule tracks are used several times

  22. Kinesins in A. nidulans • BimC Mitosis (R. Morris) • KlpA Mitosis (R. Morris) • KipB Microtubule dynamics, • Mitosis (P. Rischitor) • KinA Tip extension (N. Requena) • KipA Directionality (S. Konzack) • Two Unc104 homologues plus four • others to be studied Interphase Mitosis Interphase after septation 103 137 (240/50) 56 123 285 117 7 56 (112/16) (240/29) (292/42) Microtubule organization in A. nidulans

  23. Nuclei Mitos Nuclei Mitos BFP sGFP mRFP1 Tools Fluorescent proteins Toews et al., 2004, Curr. Genet. online

  24. High-throughput tagging (Gateway) Gene of interest Toews et al., 2004, Curr. Genet. online

  25. Anne Blumenstein Kay Vienken Alex Schütz Sven Konzack Cathrin Enke Daniel Veith Nicole Scherr Patricia Rischitor fischerR@staff.uni-marburg.de

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