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Input data for analysis. Users that have expression values (dataset 1_ chicken affy_foldchane.txt. can upload that file as shown in slide 30. More information available in IPA_workshop.doc. Input data format. Save input data as a new project including metadata. Create analysis.
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Users that have expression values (dataset 1_ chicken affy_foldchane.txt. can upload that file as shown in slide 30
More information available in IPA_workshop.doc Input data format
Analysis settings Applying filters Information about mapped/unmapped data More information available in IPA_workshop.doc
Analysis summary Export
Select and view network Right click
Functions tab More information available in IPA_workshop.doc Biological functions
Customize functions
Molecular and cellular functions only Export options
Customize pathways Statistical scoring
Coverage i.e. ratio Of pathways
Networks tab. Genes have associated fold change (decrease (green) and increase (red))
TREM1 signaling Some IPA results are available in the folder entitled IPA_results
Connect all entities in the list Select all entities
Build connections applying all desired filters More information available in IPA_workshop.doc
Interconnected genes from the list Save as a new pathway
Saved list & pathway
Acknowledgement Ingenuity Pathways Analysis (web and help manual) IPA offers weekly (Thursday) training http://www.ingenuity.com/products/training.html Additional IPA resources http://www.ingenuity.com/library/index.html