170 likes | 323 Views
Why research turtle shells?. The question,
E N D
1. River Specific Shell Morphology of Emydura Turtles Jonathan J. Reinig: jjreinig@ucdavis.edu
McNair Scholars Program
Dr. H. Brad Shaffer
Department of Evolution and Ecology
University of California, Davis
2. Why research turtle shells? The question, “What is a species?” has concerned scientists for centuries
The reasons for classifying organisms into species is profound
Knowing the amount of species that exist on our planet contributes to our basic understanding of life on earth
In order to protect biodiversity on earth we need to protect as many species as possible
If cryptic species exist, and are in danger, than we will not be able to protect them
3. Emyduras: Emydura is a genus of moderate sized, “short necked” turtles that inhabit fresh water streams and rivers Emydura maquarii
This genus of turtles belongs to the family Chelidae, which is distributed throughout Australasia, South America, and Africa (Cogger, 2000)
4. Emydura Range: Emydura is native to eastern and northern Australia, and New Guinea (Cann, 1998)
Note: this project was not concerned with specimens from New Guinea
5. The Emydura dilemma: The number of species that exist within Emydura has been under debate for some time (Arthur and Adams, 1992)
Cogger claims that the genus is composed of Five species (Cogger, 2000)
Cann, however, believes that the genus contains at least ten species, with one species, Emydura maquarii, containing seven subspecies (Cann, 1998)
This project used a technique known as Morphametrics to determine if river specific shell differences occur within the genus Emydura
The long term goal of this project is to compliment genetic data to define definitive species boundaries within Emydura
6. Morphametrics: Morphametrics is a technique used to discern minute morphological differences between organisms
It uses computer software to
place digital landmarks on pictures
of a set of organisms
The digital land marks are than analyzed via additional computer software
7. Materials: Dr. H. Brad Shaffer has compiled genetic samples and a complete photo record of approximately 1,500 Emyduras
This photo record consists of one photo of the carapace (the top shell) and one photo of the plastron (the bottom shell) for each of the 1,500 turtles
Each photo was then digitally analyzed using TPS digital software
8. Methods: carapace The carapace had 49 digital land marks placed on it
These digital land marks where placed on each turtle in the same spots of the carapace, in the
same order
9. Methods: plastron The plastron received 16 digital land marks
As with the carapace,
the digital landmarks
where placed in the
same location and
order for each
plastron
10. Analyzing the Data: Each of these landmarks represent a X and Y coordinate on a grid.
A commercial software program known as JMP was then used to analyze the data collected from the TPS digitization's
JMP was than used to test for statistical differences existing in the shell morphology of different populations inhabiting different rivers
11. Results: Currently, I’m still conducting data collection on this project
I hope to start to get results by late August
Many curious problems arose while digitizing the turtle shells
12. Unusable photos:
13. Discussion: We hope to find river and/or geographical specific shell morphologies
This would indicate that there is negligible gene flow between rivers
If this predictions holds true, than information from this project will contribute to our understanding of the true species boundaries of Emydura
The different shell types will help to define characteristics of each species
14. Next steps (Future Studies): Next month I will be traveling to Brisbane, Australia gather more photos of Emyduras
I will visit the Queensland museum, which houses some 700 Emyduras.
These will be photographed and will greatly increase our sample size
Dr. Shaffer will compare the shell morphology data with the corresponding genetic data to see if genetic clades exhibit unique shell morphology relative too each other
15. Work Cited: Cann, John. Australian Freshwater Turtles. 1998. Beaumont Publishing Pte Ltd.
Cogger, Harold G. Reptiles and Amphibians of Australia. 6th ed. 2000. New Holland Publishers (Australia) Pty Ltd
Georges, Arthur. Adams, Mark. A Phylogeny for Australian Chelid Turtles Based on Allozyme Electrophoresis. 1992 Australian Journal of Zoology, 40, 453-76
16. Acknowledgments: I would like to thank:
Dr. Brad Shaffer,
Everyone in the Shaffer lab,
The McNair program, staff, and fellow scholars
The Presidents Undergraduate Fellowship
17. River Specific Shell Morphology of Emydura Turtles Jonathan J. Reinig; jjreinig@ucdavis.edu
McNair Scholars Program
Dr. H. Brad Shaffer
Department of Evolution and Ecology
University of California, Davis