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% survival

Sensitivity to H 2 0 2 and Glutathione Levels. 100. 100. 80. 80. 60. 60. % GSH content. % survival. 40. 40. 20. 20. 0. 0. w.t. D ynk. w.t. D ynk. NDP kinase Activity. Autophosphorylation Activity. Structure and Functional Analysis of YNK. 100. 80. 60. % survival. 40.

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% survival

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  1. Sensitivity to H202 and Glutathione Levels 100 100 80 80 60 60 % GSH content % survival 40 40 20 20 0 0 w.t. Dynk w.t. Dynk

  2. NDP kinase Activity Autophosphorylation Activity

  3. Structure and Functional Analysis of YNK 100 80 60 % survival 40 20 0 w.t. Dynk Dynk + YNK Dynk + H119F Dynk + P73H Dynk + C118A

  4. Proteomix Analysis ofynk1 Knock-Out w.t. Dynk w.t. + H202 Dynk + H202 CPY: PPIase

  5. Arabidopsis

  6. Accumulation of NDPK2 Transcript during Oxidative Stress

  7. ATP GTP Enhanced NDP Kinase and Autophosphorylation Activity in Transgenic Plant Overexpressing NDPK2 transgenic w.t. transgenic w.t. H2O2 : 0 5 10 0 5 10 (mM) 0 5 10 20 30 0 5 10 20 30 (mM) NDPK activity autophosphorylation activity

  8. NDPK2 Regulates Cellular Redox Conditions wild-type transgenic knock-out 100 relative fluorescence (%) 50 0 w. t. k. o. t. g.

  9. NDPK2 Determines Phosphorylation of Two Putative Oxidative Stress Activated Proteins wild-type + H202 transgenic knock-out wild-type 47 kD 42 kD

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