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What is lactose intolerance?. Inability to digest lactose!. Catalyzed reaction. Enzyme: Substrate (reactant): Products:. Lactose Intolerance lab. In pairs, you will observe what happens when you add lactase (enzyme) to regular milk and lactose-free milk.
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Catalyzed reaction Enzyme: Substrate (reactant): Products:
Lactose Intolerance lab • In pairs, you will observe what happens when you add lactase (enzyme) to regular milk and lactose-free milk. • You will have to figure out which milk is which!
Talk to the text • Plant-Based Ice Cream Hits European Markets • Science has given ice cream a makeover, expanding the number of options for people with lactose intolerance who cannot digest most dairy products.
Review TTTT • What was special about the ice cream “Lupinesse”?
Steps • Have the partner on the left grab a green tray. ON THE YELLOW SHEET • Read the introduction and answer the pre-lab questions to prepare for the lab. • After you have answered all the questions, follow the experimental procedures.
Glucose indicator test • The glucose strips will tell you whether glucose is present in the liquid. • Dip the glucose strips in briefly so that the end gets wet with the liquid. Do not leave it in the liquid. • Wait 30 seconds and then check your results (color change) against the key provided. • Record both the color (in words) and the glucose concentration (in numbers).
Some tips! • The substances you will use are along the back counter. • Positive (+) control = glucose solution • Negative (-) control = water • A & B milk • Take your test tubes to the counter to get the substances. • Use the markings on your pipet to make accurate measurements. • Pipet = the tool like a dropper • Use the marker to crush the tablet WHILE IT IS STILL IN THE PACKET. Be careful not to puncture the packet. • Regular milk and lactose-free milk look the same, so make sure to label your test tubes. • Record your results as you go along.