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KULTUR BAKTERI -ISOLASI DAN INOKULASI-

KULTUR BAKTERI -ISOLASI DAN INOKULASI-. KULTUR BAKTERI. Used to grow bacteria Enrich the numbers of bacteria Select for certain bacteria and suppress others Differentiate among different kinds of bacteria. PURE CULTURE. Mikrobia yang dipelajari harus dalam bentuk kultur murni

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KULTUR BAKTERI -ISOLASI DAN INOKULASI-

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  1. KULTUR BAKTERI-ISOLASI DAN INOKULASI-

  2. KULTUR BAKTERI • Used to grow bacteria • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria

  3. PURE CULTURE • Mikrobia yang dipelajariharusdalambentukkulturmurni • Strain: kumpulanselkulturmurni yang berasaldarisatuseltunggal • UntukmemperolehkulturmurnimakaharusbekerjadenganTeknikAseptis • Semuabahandanalat yang digunakanharussteril

  4. PURPOSE OF CULTURING • Isolation • Menyuburkan bakteri • To create antigens for laboratory • Uji Sensibilitas • Uji Aktivitas Antibakteri • Estimate viable counts • Maintain stock culture

  5. METHODS OF ISOLATION OF PURE CULTURE • SURFACE PLATING • ENRICHMENT MEDIUM • SELECTIVE MEDIUM • INDICATOR MEDIUM

  6. METHODS TO ISOLATE THE BACTERIA • STREAK CULTURE • POUR PLATE • SPREAD PLATE • STAB • LIQUID CULTURE • SPECIAL METHODS FOR ANAEROBIC CULTURES

  7. STREAK PLATE METHOD

  8. SLANT OBSERVATION

  9. STAB CULTURE • Puncturing sutable medium such as nutrient agar, gelatin • Observe gelatin liquefaction • Preserving the stock culture

  10. LIQUID CULTURING Liquid cultures are done in: • Tubes • Bottles • Flasks (labu) • Blood culture • Water Analysis

  11. BROTH MEDIUM

  12. LIQUID MEDIA • Easiest to prepare and use • Good for growing quantities of microbes needed for analysis of experiments • Unless inoculated with pure culture cannot separate different organisms

  13. HOW TO INOCULATE A CULTURE PLATE • Plate : providege large surface for isolation and observation of colonies • Using sterile loop or sterile swab streak your sample on the petri plate • Important let your sterilized loop cool before you pickup your sample

  14. COLONIES – MAKE A OBSERVATION • SHAPE • SIZE • ELEVATION • EDGE • SURFACE • OPACITY • CONSISTENCY

  15. SWEEP PLATE METHOD

  16. ANAEROBIC BACTERIAL ISOLATION • Desicator • Candle Jar • Anaerobic Jar • Gas Pack

  17. TERIMAKASIH

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