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Activities done :

Innovative integrated methodology for the use of decontaminated river sediments in plant nursing and roadbuilding Activity of partner UniFi. Activities done :. Dissemination – done Sediment monitoring ( BioTox ) – done Microbial diversity analysis (in progress). Dissemination :.

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Activities done :

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  1. Innovative integrated methodology for the use of decontaminated river sediments in plant nursing and roadbuilding Activity of partner UniFi

  2. Activitiesdone: • Dissemination– done • Sedimentmonitoring (BioTox) – done • Microbialdiversityanalysis (in progress)

  3. Dissemination: • SICA Winter School – Piacenza, Feb., 2014 • Workshop REMIDA – ARPA Umbria, Terni, Oct., 2013 • Proceedings of the REMIDA workshop Mar 2014 • Presentation at the UniFiinstitution – Mar., 2014

  4. Sedimenttoxicitymonitoring: BioTox test The sediments toxicity analysis is assayed with the BioToxtest (AboatoxOy, Turku, Finland) according to the ISO standard method (ISO 11348e3, 1998). • Bio-indicator: Vibrio fischeri, a luminescent marine bacterium.  _ Sensitivity to contaminants  N sample bacteria _ Luminescence  amount of contaminants • Advantages: _ Standardization _Many kind of substances (soils and sediments; liquids or solids) _ Economical and rapid _ Correlation with the effects on higher organisms (Qureshi et Al., 1998)

  5. BioTox test: analysisprocedure • Suspension of 2 g of sieved (<2 mm) sediments in 8 ml of 2% NaCl • Shake for 5min by hand and settling for 30min • Adjustment of pH and conductivity • Reconstituition freeze-dried V. fischeri cells • Addition of 300 ml of the bacterial suspension to 300 ml of samples (solid/supernatant) • Measure of bioluminescence • Calculation of the inhibition of bioluminescence index (INH%) after 15 or 30 minutes < 20% INH% > 20%

  6. BioTox test: preliminaryresults Untreatedsedimentsdredged in November, 2013: pore water (toxicity of lecheates) and sedimentslurry Legend 1: Incile 2: Sandy; low contamination 3: Sandy-clay; middle contamination 4: Clay-silty;middle contamination Toxic!

  7. BioTox test: preliminaryresults Untreatedsedimentsdredged in November, 2013: dilituitionseries on 1 and 2 samples Legend 1: Incile 2: Sandy; low contamination

  8. BioTox test: preliminaryresults Treatedsedimentssampled in November 28, 2013: torbidsamples None samplesshowedtoxicity! Legend 1: Paspalumvaginatum + Eiseniafoetida 2: Tamarixgallica+ Paspalumvaginatum + Eiseniafoetida 3: Spartiumjunceum + Paspalumvaginatum + Eiseniafoetida 4: Neriumoleander + Paspalumvaginatum + Eiseniafoetida 5: Phragmitesaustralis + Paspalumvaginatum + Eiseniafoetida 6: No plants used as control area

  9. BioTox test: preliminaryresults Treatedsedimentssampled in January28, 2014: pore water (toxicity of lecheates) and sedimentslurry The last samplingconfirmed the non toxicity of the treatedsediments!

  10. Conclusions • The solidphaseof untreatedsediments (samples 1 and 2) istoxic • The pore waterisnottoxic, indicatingthatcontaminants are stronglyretained by the solidphases • Phytoremediatedsedimentsare non toxiceither the solidphase and the lecheates • Thereis no differencebetween 0-30 cm (high plantrootcolonization) and 30-60 cm (lowplantrootcolonization) • Microbialactivityseems the mainfactor in de-toxification of treatedsediments

  11. Microbialdiversityanalysis… in progress Twoaims: 1) Understandingthe progress of microbialdiversity 2) Amendphytoremediatedsediments with plantgrowthpromotingrhizobacteria (PGPR)

  12. Additionalactivities Test the performance of phytoremediatedsedimentsasa suitablesubstrate in realplant nursery companies

  13. Thanksfor yourattention!

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