与发育相关的基因和出生缺陷

1. 与发育相关的基因和出生缺陷 张咸宁 zhangxianning@zju.edu.cn Tel：13105819271; 88208367 Office: C303, Teaching Building 2015/09

2. 出生缺陷（Birth defect）：即先天性疾病 • Malformation畸形：A recognizable pattern of dysmorphic features having a single cause, either genetic or environmental(e.g., cleft lip唇裂, polydactyly多指趾).单因素（遗传或环境因素）引起的畸形 • Sequence序列征：A recognizable pattern of dysmorphic features due to a number of different causes(e.g., Pierre Robin sequence, a disorder in which a primary defect in mandibular下颌骨 development produces a small jaw, secondary glossoptosis舌后坠, and a cleft palate腭裂)多个不同因素引起的畸形 • Dysplasia发育异常：A congenital abnormality due to abnormal development at the tissue level of organization (dyshistogenesis组织发生障碍)(e.g., vascular malformation血管畸形). • Syndrome综合征：A characteristic pattern of anomalies, presumed to be causally related(e.g., trisomy 13).特定疾病的病征和症状的总合 • Deformation变形：A recognizable pattern of dysmorphic features caused by extrinsic外在的 factors that affect the fetus in utero. It usually occurs in the fetal period, not in embryogenesis胚胎发生. (e.g., oligohydramnios羊水过少) 子宫中因外部因素影响胎儿而导致的一种畸形 • Disruption畸化:A birth defect caused by destruction of tissue; may becaused by vascular occlusion, a teratogen, or rupture of theamniotic sac with entrapment(e.g., secondary limb defect resulting from a vascular event).组织破坏引起的出生缺陷；可能由血管闭塞、致畸剂或羊毛囊破裂引起

5. Developmental Gene Families • Model animals模式动物: Drosophila melanogaster, frog, mouse, chick, zebra-fish… • Transcription factors转录因子can switch genes on and off by activating or repressing gene expression. • Fundamental embryological processes: induction诱导(the process in which extracellular signals胞外信号 give rise to a change from one cell fate细胞结局 to another in a particular group of cells), segmentation分节, migration迁移, differentiation分化, and PCD (apoptosis细胞凋亡). • These processes are mediated by growth factors生长因子, cell receptors细胞受体, and chemicals (morphogens形态发生素).

6. Transcription factors转录因子 • These gene regulatory proteins调控蛋白 have a transcriptional activation domain转录激活功能域 and a DNA-binding domain DNA结合域. • 4 types of DNA-binding domain: helix-turn-helix螺旋-转角-螺旋, zinc finger锌指, leucine zipper亮氨酸拉链, helix-loop-helix螺旋-环-螺旋.

7. 1.Early Patterning早期图式发育 • Induction of the mesoderm中胚层的诱导-the initiation, maintenance, and subsequent patterning of this layer-involves several key families of signaling factors. • TheNodal family is involved in initiation, FGFs (fibroblast growth factors成纤维细胞生长因子) and WNTs (wingless) are involved in maintenance, and BMPs (bone morphogenetic proteins骨形态发生蛋白) are involved in patterning the mesoderm. • The WNT pathway has two main branches: β-catenin-dependent (canonical标准的) , independent of β-catenin. • E.g., Mutated WNT10A in man results in a form of ectodermal dysplasia外胚层发育不良症 (odonto-onychodermal dysplasia).

8. 2.The TGF-β (transforming growth factor-β) Superfamily超家族 in Development and Disease • 33 members of this cytokine family细胞因子家族. • 2 groups: (1) the BMPs, (2) the TGF-βs, activins, nodal, and myostatin筒箭毒碱, acting through various SMAD proteins. • Regulation of the cell cycle, cell migration, cell size, gastrulation原肠胚形成 and axis枢椎 specification, and metabolic processes. • In relation to health and disease, there are consequences for immunity, cancer, heart disease, diabetes, and Marfan syndrome.

9. A summary of biological responses to TGF family signaling. The range of processes that come under the influence of this super family is very broad.

10. 3.Somatogenesis（体质发生） and the Axial Skeleton（中轴骨骼） • Wnt and FGF signals play vital roles in the specification of the presomitic mesoderm前体节中胚层. • The key pathway here is notch-delta signaling and the ‘oscillation clock振荡时钟’-a precise, temporally defined wave of cycling gene expression (c-hairy in the chick, lunatic fringe and hes genes in the mouse) that sweeps from the tail-bud尾芽 region in a rostral嘴的 direction and has a key role in the process leading to the defining of somite体节 boundaries. • E.g., presenile dementia (presenilin-1), which is dominantly inherited, and spondylocostal dysostosis脊椎肋骨发育不全 (delta-like-3, mesoderm posterior-2, lunatic fringe, and hairy enhancer of split-7), which is recessively inherited.

11. Somatogenesis and Notch-Delta pathway. T-box genes have a role in PSM specification, whereas the segmentation clock depends on oscillation, or cycling, genes that are important in somite boundary formation where genes of the Notch-Delta pathway establish rostro-caudal polarity. HOX genes have a global function in establishing somite identity along the entire rostro-caudal axis.

12. 4.The Sonic Hedgehog-Patched GLI Pathway • The Sonic hedgehog gene (SHH) induces cell proliferation增殖 in a tissue-specific distribution and is expressed in the notochord脊索, the brain, and the zone of polarizing activity of developing limbs. • The key intracellular targets are the GLI family of transcription factors. • Mutations in, or deletions of, SHH (7q36) cause holoprosencephaly前脑无裂畸形; Mutations in PTCH (9q22) result in Gorlin syndrome; Mutations in GLI3 (7p13) cause Pallister-Hall and Grieg syndromes.

13. The Sonic hedgehog (Shh)-Patched (Ptch)-Gli pathway and connection with disease. Different elements in the pathway act as activators (arrows) or inhibitors (bars). The Shh protein is initially cleaved to an active N-terminal form, which is then modified by the addition of cholesterol. The normal action of Ptch is to inhibit Smo, but when Ptch is bound by Shh this inhibition is removed and the downstream signaling proceeds. CREBBP, cAMP response element-binding binding protein.

14. 5. Homeobox (HOX)同源框Genes • Proteins from homeobox-containing (or HOX) genes are important transcription factors that activate and repress batteries of downstream genes. At least 35 downstream targets are known. • Drosophila果蝇has 8 Hox genes arranged in a single cluster. • Humans, as in most vertebrates脊椎动物, there are 4 homeobox gene clusters containing a total of 39 HOX genes. • E.g., Mutations in HOXA13 cause a rare condition known as the hand-foot-genitalsyndrome 手-足-外生殖器综合征.

15. Homeobox（同源〔异型〕框）refers to nucleic acid. Homeodomain（同源〔异型〕域）refers to protein. The homeodomain is a 60 AA helix-turn-helix DNA-binding domain that is very conserved during evolution. It fits into the major groove of the DNA. Define Hox, homeobox The term homeobox is reserved for the nucleic acid sequences that encode homeodomains. Since they are highly conserved, and can be detected by low-stringencyhybridization低严谨杂交 across species.

16. Edward B. Lewis Homeotic genes specify body segment identity in Drosophila. Lewis predicted Hox genes would be duplicated.

17. A：Drosophila has eight Hox genes in a single cluster whereas there are 39 HOX genes in humans, arranged in four clusters located on chromosomes 7p, 17q, 12q, and 2q for the A, B, C, and D clusters, respectively. B：Expression patterns of Hox and HOX genes along the rostro-caudal axis in invertebrates and vertebrates, respectively. In vertebrates the clusters are paralogous and appear to compensate for one another.

18. Vertebrates have 4 Hox complexes, with about 10 genes each. They display colinearity（共线性）: a) Spatial（空间）colinearity: the more anteriorly（前部）expressed genes are in one end, the more posterior ones at the other end of the gene complex. b) Temporal（时间）colinearity: genes on one end of the complex are expressed first, those on the other (posterior。后部) end are turned on last. c) Anterior Hox genes are activated sequentially by retinoic acid. Hox genes can be aligned in 13 groups of paralogues 种内同源基因 that were duplicated twice.

19. 6. Paired-Box (PAX) Genes • The paired-box is a highly conserved高度保守 DNA sequence that encodes a 130-amino-acid DNA-binding transcription regulator domain. • 9 PAX genes have been identified in mice and humans.

21. 7.SRY-type HMG (high-mobility高泳动 group) Box (SOX) Genes • The SOX genes are transcription regulators and are expressed in specific tissues during embryogenesis胚胎发生. • E.g., SOX1, SOX2, and SOX3 are expressed in the developing mouse nervous system. • Mutations in SOX10 on chromosome 22 cause a rare form of Waardenburg syndrome.

22. 8. T-Box (TBX) Genes • This gene, which is also known as Brachyury鼠短尾突变体表型, encodes a transcription factor that contains both activator and repressor domains. • E.g., Loss-of-function mutations in TBX3 cause the ulnar尺骨-mammary乳房 syndrome.

23. 9. Zinc Finger Genes • The zinc finger refers to a finger-like loop projection突出物 consisting of a series of four amino acids that form a complex with a zinc ion. • Genes that contain a zinc finger motif act as transcription factors through binding of the zinc finger to DNA.

25. 10.Signal Transduction信号转导 ('Signaling') Genes • Signal transduction is the process whereby extracellular growth factors regulate cell division and differentiation by a complex pathway of genetically determined intermediate steps.

26. （1）The RET Proto-oncogene • Gain-of-function mutations, whether inherited or acquired, are found in a high proportion of thyroid cancers（多发性内分泌腺瘤2型/MEN 2）. • Loss-of-function mutations in RET have been identified in ~50% of familial cases of Hirschsprung disease, in which there is failure of migration of ganglionic cells to the submucosal and myenteric plexuses of the large bowel.

27. （2）FGF Receptors

28. A young child with achondroplasia软骨发育不全

29. 11.The Pharyngeal Arches咽弓 • The pharyngeal (or branchial) arches correspond to the gill system鳃系 of lower vertebrates and appear in the fourth and fifth weeks of development. • 5 (segmented) pharyngeal arches in humans arise lateral to the structures of the head and each comprises cells from the three germ layers and the neural crest.