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ENVIRONMENTAL ANALYSIS I. Solid Phase Extraction

ENVIRONMENTAL ANALYSIS I. Solid Phase Extraction. mechanised L/L extraction – ISOLUTE HM-N. ISOLUTE HM-N Liquid/Liquid Extraction Columns. ISOLUTE HM-N columns: Standard polypropylene tubes packed with ISOLUTE HM-N material (previously available in bulk only).

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ENVIRONMENTAL ANALYSIS I. Solid Phase Extraction

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  1. ENVIRONMENTALANALYSISI. Solid Phase Extraction mechanised L/L extraction – ISOLUTE HM-N

  2. ISOLUTE HM-N Liquid/Liquid Extraction Columns ISOLUTE HM-N columns: • Standard polypropylene tubes packed with ISOLUTE HM-N material (previously available in bulk only). • To aid flow under gravity, the normal top frit (hydrophobic) is replaced by a mesh retaining ring (allows sample to flow easily under gravity)

  3. How does it Work? • Modified form of diatomaceous earth. • High surface area. • Aqueous sample absorbed by the hydrophillic surface, leaving the analytes on the surface. • Non water miscible solvent is applied and liquid-liquid extraction occurs. Analytes are eluted with the organic solvent.

  4. Advantages • High surface area - very efficient extraction. • No emulsion formation. • Traditionally performed under gravity - less labour intensive than traditional liquid-liquid. • Can be automated.

  5. Configurations Description Part # ISOLUTE HM-N (0.3 ml sample) 800-0040-BM ISOLUTE HM-N (1.0 ml sample) 800-0100-CM ISOLUTE HM-N (3.0 ml sample) 800-0220-DM ISOLUTE HM-N (5.0 ml sample) 800-0350-EM ISOLUTE HM-N (10.0 ml sample) 800-0700-FM ISOLUTE HM-N (20.0 ml sample) 800-1300-FM

  6. How to Use ISOLUTE HM-N? Step 1 Apply sample Step 3 Add organic solvent Step 2 Wait for 3-5 min Sample retention area (75%) Collect extract SAMPLE SHOULD NOT COME OUT OF COLUMN!!

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