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Transcription cycle

Transcription cycle. Microcin j25. TRCF (mfd). GreA GreB. Rifampicin. Anti-  's. Activators. Transcription cycle. TRCF (mfd). Miller spread. Transcription Repair Coupling Factor (TRCF, mfd). !. Selby & Sancar (1993) Science 260 , 53. Mahdi et al. (2003) EMBO J . 22 , 724.

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Transcription cycle

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  1. Transcription cycle Microcin j25 TRCF (mfd) GreA GreB Rifampicin Anti-'s Activators

  2. Transcription cycle TRCF (mfd)

  3. Miller spread

  4. Transcription Repair Coupling Factor (TRCF, mfd) ! Selby & Sancar (1993) Science260, 53.

  5. Mahdi et al. (2003) EMBO J. 22, 724. Chambers et al. (2003) Nucleic Acids Res. 31, 6409. Selby & Sancar (1993) Science260, 53. Selby & Sancar (1995) J. Biol. Chem. 270, 4882.

  6. Rescue • Transcript cleavage (stimulated by GreA/GreB) • Slide forward

  7. Requirements for TRCF activity on the elongation complex Park et al. (2002) Cell 109, 757

  8. RecG Singleton et al. (2001) Cell 107, 79 TRCF Park et al. (2002) Cell 109, 757

  9. Alexandra Deaconescu Collaborators Anna L. Chambers, Abigail J. Smith, Nigel J. Savery (University of Bristol, Bristol, UK) Bryce E. Nickels, Ann Hochschild (Harvard Medical School)

  10. Data collection and model refinement statistics for apo Mfd Experimental electrondensity map (after solvent modification) Deaconescu et al. (2006) Cell124, 507.

  11. Deaconescu et al. (2006) Cell124, 507.

  12. Deaconescu et al. (2006) Cell124, 507.

  13. APS NE-CAT-8BM APS SBC-19ID Deaconescu et al. (2006) Cell124, 507. R/Rfree = 0.25/0.29 (3.2 Å resolution)

  14. Singleton et al. (2001) Cell 107, 79 Deaconescu et al. (2006) Cell124, 507.

  15. Truglio et al. (2002) EMBO J. 23, 2498 UvrB homology module, UvrA recruitment, and the role of D7

  16. • necessary for RNAP binding (Selby & Sancar, 1995) • binds RNAP b subunit 19-142 (Park et al., 2002)

  17. DNA-binding and ATPase activity of TRCF-L499R mutant intact Deaconescu et al. (2006) Cell124, 507.

  18. Deaconescu et al. (2006) Cell124, 507.

  19. A. L. Chambers, A.J. Smith, N. J. Savery B. Nickels, A. Hochschild Leu499 Smith & Savery (2005) Nucleic Acids Res. 33, 755 Park et al. (2002) Cell 109, 757 Park et al. (2002) Cell 109, 757

  20. Dürr et al. (2005) Cell 121, 363

  21. • TRCF - compact arrangement of structural domains linked by long, flexible linkers - appears ‘primed’ for large scale conformational changes (translocation module, UvrA binding surface). ? Nature and timing of TRCF conformational changes during the steps of TRCF-mediated transcription-coupled repair reaction? (recognition of a stalled RNAP; forward translocation of the RNAP to the transcription block; RNAP release and transcript termination; recruitment of the NER machinery to the site) ? Details of protein/protein interactions involving TRCF? (TRCF/RNAP and TRCF/NER machinery) ? Termination mechanism?

  22. Anna L. Chambers, Abigail J. Smith, Nigel J. Savery (University of Bristol) Bryce E. Nickels, Ann Hochschild (Harvard Medical School) Chris Roberts, Jeff Roberts (Cornell University) Chris Selby, Aziz Sancar (University of North Carolina, Chapel Hill) N. Sukumar , M. Capel, S. Ealick (APS NE-CAT-8BM) A. Joachimiak, S. Grinnell (APS SBC-19ID) Darst Lab Matthew Bick Elizabeth Campbell Linda (Madge) Champagnie Alexandra Deaconescu Raji Edayathumangalam Deepti Jain Valerie Lamour William Lane Fred Leon Natacha Opalka Georgia Patikoglou Sheng Wang Lars Westblade Kelly-Anne Wilson

  23. Bacterial transcription termination Figure 1 A. Intrinsic terminator B. Rho C. Mfd

  24. Roadblock repression assay (Chambers et al., 2003, Nucleic Acids Res. 31, 6409)

  25. DNA-binding activity of TRCF-L499R mutant intact (also ATPase activity)

  26. Epshtein & Nudler (2003) Cooperation between RNA polymerase molecules in transcription elongation, Science300, 801.

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