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t otal RNA Pufication ( 2.9.1 ). cDNA. In-Vitro Transcription. Total RNA. Quality control ( 2.9.2.1). Incubation for 22 h at 37°C. 500 ng total RNA. 1 st strand cDNA synthesis. cRNA cleanup. Reverse trascription for 2 h at 42°C. Agencourt RNAClean XP. 2 nd strand
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total RNA Pufication (2.9.1) cDNA In-Vitro Transcription Total RNA Quality control (2.9.2.1) Incubationfor 22 h at 37°C 500 ngtotal RNA 1ststrand cDNAsynthesis cRNA cleanup Reverse trascriptionfor 2 h at 42°C Agencourt RNAClean XP 2ndstrand cDNAsynthesis cRNA Quality control (2.9.2.1) Incubationfor 2 h at 16‘°C 750 ngcRNA in GEX-HYB buffer cDNA cleanup Hybridization (2.9.3.2) Agencourt RNAClean XP
