Total RNA Sequencing

1. Total RNA Sequencing Total RNA-Seq analyzes offer a comprehensive view of the transcriptome. A range of solutions tailored to your study purpose is available at CD genomics. We are able to provide fast reliable and cost- effective service. As noncoding RNA (ncRNA) continues to be recognized of biological importance, total RNA-Seq analyzes both coding and multiple forms of noncoding RNA for a comprehensive view of the transcriptome. Global analysis of RNA expression can enhance our understanding of all transcriptional activity. Total RNA-Seq enables analysis of coding and noncoding RNA with the high-confidence discovery of features such as alternative transcripts, gene fusions, allele-specific expression, and the detection of novel transcripts in both coding and non-coding RNA species. CD genomics combines proven ribosomal reduction library preparation chemistries and illumina NGS technology into a single, streamlined protocol. Ribo-Zero ribosomal RNA reduction chemistry minimizes ribosomal contamination and maximizes the percentage of uniquely mapped reads. With the consistent robust performance, total RNA-seq covers both mRNA and a broad range of ncRNA species of interest, including long noncoding RNA (lncRNA), small nuclear (snRNA), small nucleolar (snoRNA), and other RNA species. Advantages of total RNA Sequencing Precise measurement of strand orientation and uniform coverage. Compatible with multiple sample types including low-quality, formalin-fixed, paraffin-embedded (FFPE) Fast turnaround time and highest data quality Lower cost and wide availability. Effective transcriptome analysis      Total RNA Sequencing workflow Our comprehensive RNA-Seq services provide the RNA sequencing workflow from sample preparation through data analysis, enable rapid profiling and deep insight of the RNA.

2. Sample Requirements Total RNA amount should be more than 5 μg. Recommended concentration should be 20ug/uL or more. A260:A280 value should be between 1.8 and 2.0 RNA should be stored in nuclease-free water or RNA Stable. RNA must be validated using an assay such as a BioAnalyzer RNA chip. RIN value ≥ 7.0 Tissue, cell, blood, and blood products are acceptable.       Sequencing Illumina High throughput sequencer Flexible service options：single end or paired-end sequencing, optional reads number according to research goals.   Data analysis Raw data QC Alignment and TPM/RPKM/FPKM-based quantitation Expression analysis Statistics of SNPs/Indels Alternative splicing analysis GO and KEGG annotation       Source: https://www.cd-genomics.com/total-rna-sequencing.html