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A Perfusion-based Micro 3-D Cell Culture Platform

A Perfusion-based Micro 3-D Cell Culture Platform. NTHU MSCL Adviser: C. H. Liu C. He Yeh. A Perfusion-based Micro 3-D Cell Culture Platform. Outline Culture environment at large scale Culture environment at micro scale Principle Fabrication Conclusion & Features

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A Perfusion-based Micro 3-D Cell Culture Platform

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  1. A Perfusion-based Micro 3-D Cell Culture Platform NTHU MSCL Adviser: C. H. Liu C. He Yeh

  2. A Perfusion-based Micro 3-D Cell Culture Platform • Outline • Culture environment at large scale • Culture environment at micro scale • Principle • Fabrication • Conclusion & Features • Further work

  3. General Culture Equipment • 細胞培養設備 • (1) 高溫高壓滅菌爐 • (2) 無菌室 • (3) 無菌操作台 • (4) 二氧化碳培養箱 • (5) 細胞離心機 • (6) 倒立顯微鏡 • (7) 恆溫水浴槽 • (8) 冷凍冷藏冰箱 • (9) 細胞計數器及血球計數盤 • (10) 本生燈 • (11) 液氮細胞儲存桶 • (12) 酸鹼測定儀 • (13) 真空幫浦 • (14) 瓦斯桶 • (15) 二氧化碳桶 Lab On a Chip

  4. On Chip Advantages • Small dimension • Saving sample • Rapid assay process (micro dimension, avoid medium or reagent diffusion time gradient or concentration different ) • Cheap, disposable

  5. On Chip Culture Requirement • Temperature : 37℃ • pH : 7.2 ~ 7.4 ( 95% air 5%CO2 orHEPES) • Buffered saline (PBS, PBSA, …etc) • Medium : (Serum, glucose, amino acids, vitamin, antibiotics ) • 3D culture (In vivo Culture)

  6. A Perfusion-based Micro 3-D Cell Culture Platform

  7. Working Principle :

  8. A Perfusion-based Micro 3-D Cell Culture Platform • Fabrication :

  9. Conclusion • Feature 1: 3D well-defined culture environment • mimic an in vivo environment • distinct cellular behaviors (cell-cell and cell-extracellular interactions ) • provide more meaningful data

  10. Conclusion • Feature 2 : Short Loading cell channel • Feature 3 : High throughput (8 chambers)

  11. Further work • Bioassay (inject reagent instead of culture medium in vivo bioassay) • Biochemical experiment • Tissue engineering • Drug screening • μTAS

  12. Reference • “A PERFUSION-BASEDMICRO 3-D CELL CULTURE PLATFORM” S. B. Huang1, M. H. Wu2, Z. F. Cui3, Z. Cui2, J. L. Lin4 and G. B. Lee1* • “MICROFLUIDIC DEVICES FOR THE ASSEMBLY AND CULTURE OF THREE-DIMENSIONAL MULTI- CELLULAR CONSTRUCTS WITH DIFFUSION-LIMITED MICROENVIRONMENTS” M. Pinelis1, R. W. Kasinskas2, R. T. Borno1, J. H. Park1, E. Chu3, N. S. Forbes2 and M. M. Maharbiz1 • "Differentiation of human Embryonic Stem Cells into Hepatocytes in 2D and 3D culture system in vitro" The International Journal of Developmental Biology, Vol. 50, pp. 645-652, 2006 • "The multilayered postconfluent cell culture as a model for drug screening" Critical Reviews in Oncology/Hematol, Vol. 36, pp. 147-157, 2000. J. M. Pardon; C.L. van der Wilt; K. Smid; E. Smitskamp-Wilms; H.H. Baukus; P. E. Piazo; G. • "Development of PDMS Microbioreactor with Well-defined and Homogenous Culture Environment for Chondrocyte 3-D Culture“ M. H. Wu, J. P. G. Urban, Z. Cui and Z. F. Cui,Biomedical Microdevices, Vol. 8, pp. 331-340, 2006.

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