1 / 10

Gel Electrophoresis

Gel Electrophoresis. DNA Unit . Rationale. Present the topics of cell function, DNA and the applications of DNA analysis in science DNA is a defined fingerprint to each individual human I ntroduce the students to occupations that use DNA analysis. Learning Outcomes.

meriel
Download Presentation

Gel Electrophoresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gel Electrophoresis DNA Unit

  2. Rationale • Present the topics of cell function, DNA and the applications of DNA analysis in science • DNA is a defined fingerprint to each individual human • Introduce the students to occupations that use DNA analysis

  3. Learning Outcomes • Students will label the different parts of the cell and define cell functions. • Students will analyze the structure of DNA; then describe how the body uses DNA. • Students will learn compare gel electrophoresis to a variety of DNA analysis and research how to use it DNA analysis in modern science.

  4. Saskatchewan Objectives • To enable students to understand and use the vocabulary, structure and forms of expression which characterize the study of biology. • To develop a contemporary view of technology. • To develop compassionate, empathetic and fair-minded students who can make positive contributions to society as individuals and as members of groups.

  5. Topic Sequence Cells & Their Function DNA & Replication GelElectrophoresis

  6. Cells and their Function Main Points • Plants vs.Animal cells • Parts of the cells(plant and animal) then compare the two • Functions of the cell and the functions of the cell parts • Grade Dependant: • Incorporate photosynthesis and Calvin Cycle • Photosynthesis- light and dark reactions • Metabolism- chemical pathways to produce ATP

  7. DNA Replication Main Points: • DNA is unique to each individual • Hereditary – light introduction • Where DNA replication takes place • What parts of the cell participate • Types of RNA • Codon and Anti codon • Proteins

  8. Gel Electrophoresis • Main Points: • DNA is denatured through boiling and DTT • The proteins that are analyzed • Voltage pushes the proteins down the gel • Uniform charge of the proteins from the SDS • Proteins separated by size • 4-20% gels • Gels should be ran on 120 volts for 1 hr or 90 volts for 1 ½ hrs… to high of voltage results in melted gels

  9. Sample Preparation and Gel Set Up

  10. Assessment • Hand outs - draw and label the cell - functions of the cell • Make a movie , audio or storyboard of DNA replication • Starting with a broad perspective use your vocabulary to focus the topic to how DNA effects humanity. • Gel electrophoresis lab • Lab notebooks – write a lab • Research uses of gel electrophoresis • Exam

More Related