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Fig. 1

Fig. 1. P-Tyr 705. P-Tyr 705. P-Ser 727. P-Ser 727. 3. 3. *. *. *. *. *. *. *. *. 2. 2. P-STAT3 Level. P-STAT3 Level. 1. 1. 0. 0. 1.0. 2.0. 0.9. 0.9. 2.1. 2.1. 1.0. 2.3. 1.1. 1.0. P-Tyr 705. P-Tyr 705. 1.0. 2.1. 1.0. 1.1. 1.0. 2.1. 1.1. 1.1. 2.2. 2.0.

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Fig. 1

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  1. Fig. 1 P-Tyr705 P-Tyr705 P-Ser727 P-Ser727 3 3 * * * * * * * * 2 2 P-STAT3 Level P-STAT3 Level 1 1 0 0 1.0 2.0 0.9 0.9 2.1 2.1 1.0 2.3 1.1 1.0 P-Tyr705 P-Tyr705 1.0 2.1 1.0 1.1 1.0 2.1 1.1 1.1 2.2 2.0 P-Ser727 P-Ser727 STAT3 STAT3 Rp DB CTX H-89 Cica Cica Basal Basal Basal Vehicle Cay10449 A B Cica

  2. Fig. 2 P-Ser727 C * # * 800 * * 4 * * * 3 * 600 * P-STAT3 Level * * * 2 Luciferase activity (Fold Stimulation) 1 400 Avg.(IP/Total)1000 1.0 2.6 0.9 2.3 1.3 2.4 1.4 * * 0 1.0 2.3 1.2 2.4 1.1 2.2 1.0 200 P-Tyr705 P-Ser727 0 STAT3 Gaq Ga11 Ga16 Ga14 Gaq Ga11 Ga16 pcDNA1 pcDNA1 GaqRC Ga11QL Ga16QL pCDNA1 Gaq Ga11 Ga16 GaqRC Ga11QL Ga16QL pcDNA1 hIPR P-Ser727 P-Tyr705 P-Tyr705 * 3 * * * * * 4 2 P-STAT3 Level 3 1 2 0 P-Tyr705 1.0 1.1 3.8 1.0 3.6 1.4 3.9 1 1.0 1.2 3.5 1.3 3.0 1.4 3.2 P-Ser727 STAT3 0 KN62 KN92 Cal.C Basal Vehicle U73122 U73343 Cica B A 10 nM Cica Basal D

  3. Fig. 3 * 3 * * 2 P-Src/P-JAK2 Level 1 Src JAK2 0 0 15 30 45 1.0 1.0 1.7 1.9 2.8 P-Src Src 1.0 1.4 2.0 1.9 2.0 P-JAK2 JAK2 0 5 15 30 45 Time (min) P-Tyr705 P-Ser727 P-Tyr705 P-Ser727 * * 3 3 * * * * 2 2 P-STAT3 Level P-Akt Level 1 1.0 1.8 2.0 1.9 1 1.0 2.3 2.2 2.6 2.4 1.4 1.4 2.2 0 1.0 2.4 1.0 1.1 0 1.0 2.7 1.2 1.1 2.4 1.5 1.5 2.3 0 15 30 45 P-Tyr705 1.0 1.2 1.7 2.6 2.8 P-Akt P-Ser727 Akt STAT3 0 5 15 30 45 Wort Basal LY294 Vehicle Time (min) Cica A B * 3 * * * * * * * 2 P-STAT3 Level 1 0 P-Tyr705 P-Ser727 STAT3 PP1 PP2 PP3 WHI Basal AG490 WHI-ve Vehicle Cica C D

  4. Fig. 4 P-Ser727 P-Tyr705 * * * * * * 3 * * * 2 P-STAT3 Level 1 0 P-Tyr705 1.0 1.0 2.5 1.0 1.0 2.6 2.7 1.1 2.6 1.2 2.3 1.1 2.4 P-Ser727 STAT3 1.0 1.0 2.2 0.9 1.1 2.5 2.6 1.1 1.2 1.2 1.1 1.2 1.1 GasQL Gas Ga16 PP1 PP2 U0126 Vehicle pcDNA1 pcDNA1 Gas + Ga16 Ga16QL P-Tyr705 P-Ser727 P-Tyr705 P-Ser727 * * 3 * * 3 * * * * * * * * * 2 2 P-STAT3 Level P-STAT3 Level 1 1 0 0 1.0 2.0 2.0 0.8 2.0 0.8 2.2 1.1 2.1 1.0 2.3 2.2 1.2 2.5 1.4 2.5 1.4 2.5 P-Tyr705 P-Tyr705 1.0 2.1 2.0 1.1 1.0 1.0 0.9 0.9 0.9 1.0 2.4 2.6 0.9 0.8 0.9 1.0 1.3 1.2 P-Ser727 P-Ser727 STAT3 STAT3 DB Cica PP1 PP2 PP1 PP2 Basal Basal U0126 U0126 Vehicle Vehicle C5a C5a A B C

  5. Fig. 5 P-Ser727 P-Tyr705 * * * * * P-Tyr705 P-Tyr705 P-Ser727 P-Ser727 * * 3 * * * * * * * 3 3 * * * * * * * * * * * * * * * * 2 * P-STAT3 Level * 2 2 P-STAT3 Level P-STAT3 Level 1 1 1 1.0 1.0 2.5 1.0 1.0 2.7 2.6 2.6 2.3 2.4 2.4 0 0 0 P-Tyr705 1.0 1.9 2.2 1.9 2.0 1.9 1.9 1.0 2.0 2.0 2.0 2.5 2.3 2.4 P-Tyr705 P-Tyr705 1.0 1.0 2.2 0.9 1.0 2.5 2.4 2.4 1.2 2.5 1.2 P-Ser727 1.0 2.3 2.6 2.6 1.4 2.4 1.4 1.0 2.1 2.0 2.0 1.4 2.3 1.4 P-Ser727 P-Ser727 STAT3 STAT3 STAT3 GasQL Cica DB Gas Ga16 Basal Wort Basal Wort Wort pcDNA1 pcDNA1 LY294 LY294 Vehicle LY294 Vehicle Vehicle Gas + Ga16 Ga16QL C5a C5a A B C

  6. Fig. 6 1.0 1.1 0.7 0.6 0.05 0.9 0.9 0.9 0.9 Gas b-Actin Ctrl 0 nM 0 nM 100 nM 200 nM 100 nM 200 nM 300 nM 300 nM siGas siCtrl

  7. Fig. 7 P-Ser727 P-Ser727 P-Tyr705 P-Tyr705 3 * * * * * * * * * * * * 2 P-STAT3 Level 1 0 1.0 2.1 1.4 1.3 2.0 2.0 1.1 1.9 1.9 2.0 2.0 1.9 P-Tyr705 1.0 1.9 1.2 1.2 2.0 2.0 1.0 1.9 1.3 1.3 1.4 1.4 P-Ser727 STAT3 PP2 PP2 Wort Wort Basal Basal U0126 LY294 U0126 LY294 Vehicle Vehicle Cica Cica siGas siCtrl 3 * * * * * * * * * * * * 2 P-STAT3 Level 1 1.0 2.0 2.1 0.9 1.9 1.2 1.8 1.7 2.0 2.0 2.0 0 P-Tyr705 1.0 2.0 2.1 1.2 1.3 1.2 1.2 1.9 1.5 2.0 1.4 P-Ser727 STAT3 DB PP2 Basal Wort U0126 LY294 Vehicle C5a A B

  8. Fig. 8 # 3 * * * * 2.5 * * * 2 * * Luciferase Activity (Fold Stimulation) * * * * 1.5 1 0 Gas PP1 PP2 PP3 WHI Ga16 Wort LY294 LY303 U0126 U0124 AG490 GasQL WHI-ve Vehicle Ga16QL pcDNA1 Ga16+Gas Ga16QL+GasQL

  9. Fig. 9 U0126 AG490 H-89 Ga16/Gas Ga16 Gas U73122 U73122 Cal C Cal C KN62 KN62 PP1/2 PP1/2 U0126 Wort AG490 Ser727 Tyr705 Ser727 Tyr705 P P P P P P hIP H-89 PLCb/PKC AC/PKA PLCb/PKC & AC/PKA Wort PP1/2 AG490 Src/JAK PI3K/Src/JAK PI3K/Src/JAK U0126 MEK/ERK MEK/ERK MEK/ERK Ser727 Tyr705

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