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Pre Lab Definitions: (Fill these in on your lab paper)

Pre Lab Definitions: (Fill these in on your lab paper). Serial: In a series, order or interval. Measured steps. Dilution: Water Down. Pipette: “Little pipe” for moving and measuring liquids. Serial dilutions reduce the concentration of a sample in small steps or fractions.

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Pre Lab Definitions: (Fill these in on your lab paper)

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  1. Pre Lab Definitions: (Fill these in on your lab paper) • Serial: In a series, order or interval. Measured steps. • Dilution: Water Down. • Pipette: “Little pipe” for moving and measuring liquids. • Serial dilutions reduce the concentration of a sample in small steps or fractions. • Lab equipment for today: Lab Coat, gloves & glasses

  2. Lab:Fill in the instructions as we go along. Read each step carefully. 1. Label test tubes1, 2, 3, 4.

  3. Lab:Fill in the instructions as we go along. Read each step carefully. • Add 10 mL of water to test tube 1. • Add 9 mL of water to test tube 2 and 3. • Add 2 mL of water to test tube 4. 5. Add 6 drops of methylene blue to test tube 1. (mix)

  4. Lab:Fill in the instructions as we go along. Read each step carefully. • Pipette 1 mL of water from test tube 1 to test tube 2. (mix) • Pipette 1 mL of water from test tube 2 to test tube 3. (mix) 8. Pipette 1 mL of water from test tube 3 to test tube 4. (mix)

  5. Post Lab Analysis • You will use this same procedure to dilute yeast samples to culture cells on petri dishes. • How could you identify the test tubes if the numbers were removed? Darkest (1) to lightest (4) • Hold your test tubes up to the light to see the color differences. What does dilution mean? • To add water; water down.

  6. Post Lab Analysis • Calculate the percentage of methylene blue in each test tube. Show your work. • 20 drops=1 mL • Test tube #1: What was in test tube #1? 10 mL of water= 10 X 20 = 200 drops 6 drops of dye 200 +6= 206 drops total in test tube #1 6 ÷ 206 X 100 = _______%

  7. Post Lab Analysis • Test tube #2: #1’s answer X .10 = ______% • Test tube #3: #2’s answer X .10 = ______% • Test tube #4: #3’s answer X .03 = ______%

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