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The Use of Fluorosurfactant FC-129 in Micellar Electrokinetic Capillary Chromatography

The Use of Fluorosurfactant FC-129 in Micellar Electrokinetic Capillary Chromatography. Ronny de Ridder 1,2 , Francesco Damin 1 , Jetse Reijenga 2 and Marcella Chiari 1 Institute of Biocatalysis and Molecular Recognition, C.N.R., Milano, Italy

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The Use of Fluorosurfactant FC-129 in Micellar Electrokinetic Capillary Chromatography

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  1. The Use of Fluorosurfactant FC-129 in Micellar Electrokinetic CapillaryChromatography • Ronny de Ridder1,2,Francesco Damin1,Jetse Reijenga2 andMarcella Chiari1 • Institute of Biocatalysis and Molecular Recognition, C.N.R., Milano, Italy • Laboratoryof Instrumental Analysis,Eindhoven University of Technology,The Netherlands

  2. FC-129 N-ethyl-N-[(heptadecafluorooctyl)sulfonyl]glycine, K salt [CAS 2991-51-7], Aldrich (Steinheim, Germany)

  3. CMC determination CMCFC-129 = 0.5 mM CMCSDS = 8 mM

  4. Spectra-Phoresis 1000TM (Thermo Separation Products, Freemont, CA, USA) Fused silica, 50m ID, 375 mOD Lt=400 mm, Ld=320 mm, V=20 kV UV detection @ 200 nm Injection 5.17.103 N/m2pressure, 4 s BGE: 12.5 mM Sodium tetraborate pH 9.9 Experimental

  5. Results EOF and Micelle Mobility

  6. Results Homologue phenol series

  7. Results 2-, 3- and 4-ethylphenol

  8. Results retention factors

  9. Conclusions • EOF- & Micelle mobility comparable • systematic decrease in retention factor • interesting selectivity differences

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