Learning About an HIV Cure by Doing Studies in HIV Subjects Alan Landay, PhD Professor and Chairman Department of Imm

2. Can evaluate natural course of HIV disease without having to use multiple primate species and SIV strains Viral set point differs between human and primate  Primates fail to reach maximal viral suppression with current ARV as compared to HIV+ subjects

3. In Vitro Assay Setting Virologic assays for evaluating HIV reservoirs following therapeutic intervention have been established for HIV+ subjects Lack cell lines from primates for latency studies and validation of human cell lines, primary human cell models and patient PBMC.

4. HIV RNA expression ishigher at all gut sites than in PBMC

5. Clinical Setting Demonstrated evidence of a �cure� in Berlin patient Gene therapy and pharmacologic agents are being evaluated in ongoing human studies Novel agents used in other clinical settings (i.e. autoimmune disease, transplant, cancer) can be adapted for cure studies in HIV+ subjects Primate models utilize small numbers of animals making interpretation of the data and its statistical significance difficult

7. HIV+ SUBJECTS CAN PROVIDE THE BEST INFORMATION FOR HIV CURE RESEARCH

8. Acknowledgements Rush Seema Desai UCSF Joe Wong UNC David Margolis