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381 PHT Lab 2

2. STAINING. 3. Staining of Bacteria. Bacteria cells are almost colorless and transparentA staining technique is often applied to the cells to color them ? Their shape and size can be easily determined under the microscope. . 4. Principle of staining. Stains ? combine chemically with the b

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381 PHT Lab 2

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    1. 1 381 PHT Lab #2

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    21. 21 Crystal violet ? Iodine ? Alcohol ? Safranin

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    26. 26 Gram-positive bacteria Have a thick peptidoglycan layer surrounds the cell. The stain gets trapped into this layer and the bacteria turned purple. Retain the color of the primary stain (crystal violet) after decolorization with alcohol Gram-negative bacteria have a thin peptidoglycan layer that does not retain crystal violet stain. Instead, it has a thick lipid layer which dissolved easily upon decoulorization with Aceton-Alcohol. Therefore, cells will be counterstained with safranin and turned red.

    27. 27 Gram Stain Materials:- Cultures of Staphylococcus aureus, Candida albican, Bacillus subtilis, E.coli Gram stain: Crystal violet (primary stain) Gram’s iodine (mordant) Acetone-alcohol (decolorizing agent) Safranin (counter stain)

    28. 28 Gram Stain Procedure:

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