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Supplemental Digital Content 2. A. i. ii. Phase contrast. CMTMR. iii. iv. Dragon Green. Dragon Green + CMTMR + DAPI. B MSC. C BMMC.
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Supplemental Digital Content 2 A i ii Phase contrast CMTMR iii iv Dragon Green Dragon Green +CMTMR+DAPI B MSC C BMMC
Figure 2. Efficiency of SPIO nanoparticle loading into MSC and BMMC. (A) Micrographs of MSC showing incorporated SPIO by (i) phase contrast, (ii) CMTMR fluorescence, (iii) Dragon-Green fluorescence and (iv) a merged image of Dragon Green, CMTMR and DAPI nuclear label. Flow cytometric analysis of (B) MSC and (C) BMMC labeled with CMTMR (left panels) and Dragon Green-SPIO (centre panels). Proficient loading of MSC and BMMC with both SPIO nanoparticles and CMTMR was confirmed by multi-channel flow cytometry (right panels), which revealed >90% Dragon Green-SPIO and CMTMR colabeled cells.