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Supplemental Digital Content 1 Fig. 1: Analysis of putative transcription factor binding sites in the ABCC4 promoter fragment. Selected putative transcription factor binding sites in the human ABCC4 proximal regulatory region (identified using Genomatix Software Suite) as well as the position of the promoter luciferase construct, which was used in this study, is indicated. Abbreviations: Ahr: aryl hydrocarbon receptor; CREB: cAMP-responsive element binding protein; Luc+: luciferase gene; Nrf2: nuclear factor-E2;
Fig. 2: Effects of treatments on cell proliferation.SMC and M07e cells were cultured in 96-well plates and 100 µM db-cAMP (+ 100 µM IBMX), 300 µM 8-CPT or db-cAMP + IBMX in the presence of the inhibitors KT5720 (10 µM) or PD98059 (25 µM) as well as BrdU(10 µM)was added for 24 h.The plates were washed, and a chemiluminescentBrdU cell proliferation assay was performed according to the manufacturer’s instructions (Roche). Similarly to the results in M07e cells, a slight decrease in cell proliferation after addition of db-cAMP was observed in HeLa cells (not shown). (mean values ± SD; un-stimulated control =100%; n= 5).
