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*. 0.10. D272NRNase R. 0.08. D280NRNase R. D. CsdA-. C. 0.06. Fraction unwound. 0.04. 0.02. 0.00. 0. 5. 10. 15. 20. 25. Time (min). Supplemental Fig. S1 Awano et al. A. CsdA- D C. RNase R-WT. D272NRNase R. D278NRNase R. D280NRNase R. Time (min).
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* 0.10 D272NRNase R 0.08 D280NRNase R D CsdA- C 0.06 Fraction unwound 0.04 0.02 0.00 0 5 10 15 20 25 Time (min) Supplemental Fig. S1 Awano et al A CsdA-DC RNase R-WT D272NRNase R D278NRNase R D280NRNase R Time (min) SS -P 0 1 3 5 10 15 20 0 1 3 5 10 15 20 0 1 3 5 10 15 20 SS -P 0 1 3 5 10 15 20 0 1 3 5 10 15 20 B
10bp * 3' 5' 5' 20 U+ 8-nt 3' 0.8 D CsdA C D272NRNase R 0.6 0.4 Fraction unwound 0.2 0.0 0 5 10 15 20 25 Time (min) Supplemental Fig. S2 Awano et al A CsdA-DC RNase R-WT D272NRNase R Time (min) ss 0 1 3 5 10 15 20 0 1 3 5 10 15 20 0 1 3 5 10 15 20 -P B