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6.3 Genetics and Predictions. You will be able to:. Explain how probability is used in genetic predictions To construct a Punnett Square for monohybrid and dihybrid crosses To infer genotype by using a testcross. Probability. the measure of how likely an event is.
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You will be able to: • Explain how probability is used in genetic predictions • To construct a Punnett Square for monohybrid and dihybrid crosses • To infer genotype by using a testcross
Probability • the measure of how likely an event is. • Measure how likely a species will inherit a trait
Represent probability • Fraction ½ • Percent 50% • Ratio (1:2)
Punnett Square • a simple graphical way of discovering all of the potential combinations of genotypes that can occur in children, given the genotypes of their parents. • It also shows us the odds of each of the offspring genotypes occurring.
Sample Problems • In pea plants , tall pea plants are dominant over short pea plants. • Using Punnett Squares, you can predict the genotypes and phenotypes of the offspring of a cross between a homozygous (purebred) tall pea plant and a homozygous (purebred) short pea plant.
You begin by drawing a grid Step 1
Designate letters which will represent the genes/traits. Capital letters represent dominant traits lowercase letters represent recessive traits. T = tall t = short Step 2
Write down the genotypes (genes) of each parent. These are often given to you or are possible to determine. TT X tt Step 3
List the genes that each parent can contribute. Parent 1 : T Parent 2: t Step 4
Write the possible gene(s) of one parent across the top The gene(s) of the other parent along the side Step 5
Fill in each box of the Punnett square by transferring the letter above and in front of each box into each appropriate box. As a general rule, the capital letter goes first and a lowercase letter follows. Step 6
Step 7 • List the possible genotypes and phenotypes of the offspring for this cross.
Monohybrid Cross • only one trait is crossed. • A tall pea plant TT is crosses with a short pea plant tt.
Dihybrid Cross • Two traits are crossed. • A tall, green flowered plant is crossed with a short, yellow flowered plant. • TtPp x ttpp
Test Cross • A simple method to confirm the genotype of the F1 hybrid. • F1 hybrid is crossed with the homozygous recessive
Test Cross Example • Tall x Dwarf • Tall Parent = F1 (Tt) • Dwarf Parent = homozygous recessive (tt)
If the F1 tall is heterozygous with (Tt) genotype • and, if Mendel's law of segregation of characters is correct, • then the F1 should produce two types of gametes, (T) and (t) in equal proportion. • The recessive dwarf parent produces only (t) type of gametes. • The resulting test cross progeny should be 50% Tall and 50% Dwarf or in 1:1 ratio as follows
The resulting test cross should be • 50% Tall and 50% Dwarf • 1:1 ratio • 1 tall for every 1 dwarf