1 / 6

Act, UV

A. Act, 6h. Act, UV. Act, 3h. con. con. UV. trex1. gapdh. 1 0.4 0.1 1 2.8 0.7. IF. B. C. col AP-1. p21 p53. 0. 5x. 10x. 50x. 0. 5x. 10x. 50x. fold excess. specific shift. ß-Gal activity. 7.5J/m 2. 20 J/m 2. control. unbound oligo. MMS. TCDD. D.

mikaia
Download Presentation

Act, UV

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. A Act, 6h Act, UV Act, 3h con con UV trex1 gapdh 1 0.4 0.1 1 2.8 0.7 IF B C col AP-1 p21 p53 0 5x 10x 50x 0 5x 10x 50x fold excess specific shift ß-Gal activity 7.5J/m2 20 J/m2 control unbound oligo MMS TCDD D mM nM Time (h) con 3 0.1 3 0.5 3 con 6 0.1 6 0.5 6 con 3 1 3 5 3 con 6 1 6 5 6 trex1 gapdh MNNG IR µM Gy Time (h) con 3 5 3 25 3 con 6 5 6 25 6 con 3 0.5 3 2 3 con 6 0.5 6 2 6 trex1 gapdh B(a)P H2O2 µM mM Time (h) con 3 2.5 3 10 3 con 6 2.5 6 10 6 con 3 0.25 3 1 3 con 6 0.25 6 1 6 trex1 gapdh Supplement 1

  2. mTrex1 Promoter CTGAGGGCCTGAGCATCCAGCCACAAGGTGGCGCCAGCTCCCACCGCCGTCTTTGCCATATCCACTGATTAAAACTGCACTTCCTGCTGAGAACACAAGCTTTGTTTCCTGAGTCCGATTTGTGTGGCTGAGAGGGAGGGACAGGGCAGACCAAGAATTGATGAGATGACTCCTGCTGGAGTTGAGATGGCCCCCATGGTAACTGATCTGCCCTGAGGAAAAACCCTGCCCCTCTTCGGGAATTACCCTGAGTCATAGCTTTGGGCGGGGGCCGTGGTGGGGGCAGGGGTACGTTTGTGGCCAAGGAGGCAGCCCTTTTTAGGAAGGCCTGGATCAAGGACTGGGGGAAAGACAAGAAAAGGCAGAGGCTCACGGGCACATTTGAATCCTAGAGACAACCCAGTTTGAGTTGTCAGTGTTGGGGCAGTAGTCCATTTTACCAGCCAGCAGGATCACAGATATGGATCTAGGAAGGCCTTTGGGGTGCTTGGGGAGGGAGGGAGGAGCGTCAGCACGAGGGCCAGGCCTGGCAGGCTAACAGCAGGGCGGGCCCTGTTCAGGAGGCAGGCCTATGGGTGGACCCAGGCAGTTTTACTTCCTCAGCCACACTGCTGCCAGCAGCAGACCCAGCGGAGTGCCTTGCTGCCTGCTTCTCGGTAAGTGTGGAGTAAGGGTATGGGGTAAGGCAGAAGAGAATAGGGTTCCCTCACCCTGAAGGTAGTCAGCACTAGGGGAAGGGCCTCCCTCAAAAGGCAAATAAGTAGTGGATCCATCCCACTAGAACAACCCTGCCCTCCAGCTTCTAGTGGCCCTGGATAGTACCTGCTGAAGGGAAGGGCTGAGCTGCCTTTCTGCACCCCACCTCAGCCCACTTGAGACCTTCCTGCTGCTACTCATTACCCCATCTCCTCCCCAGGCTCAGCATGGGCTCACAGACCCTGC GR AGAACA AGTACC WT GTGTGGCT GAGGGAGGG GCGGGGGCC p53 TGAGATGGCC NF-AT GGAAAAAC SP1 CCCTGCCCC GGGGCAGGGG GGGCGG NFκB TCCCCA TGGGGA AP1 CTGAGTCA TACTCA Ref1 TGAGAC RXR TGCCCT TCACCC GGGTGG AGGCCT c-Maf TCAGCA underlined: primer usedforcloning Supplement 2

  3. A Act-D UV Act-D con UV trex1 gapdh B col AP-1 htrex1 AP-1 col AP-1 htrex1 AP-1 0 4 8 16 0 4 8 16 time(h) 0 4 8 16 0 4 8 16 UV B(a)P Supplement 3

  4. hTrex1 5 CTGGCCTTGTTTCCTGAGTCTGATCTGTTTGGCGGAGTGGGAGGGGTGGAGCAGGACCCG mtrex1 96 CAAGCTTTGTTTCCTGAGTCCGATTTGTGTGGCTGAGAGGGAGGGA-----CAGGG--CA * ** ************** *** *** **** *** ******* **** * hTrex1 65 GACCCTGAGTGGCTGGGATCCTTCTTCCTGTCCCTGGCTGTTGCTGAGCCCGTCCCCATG mtrex1 149 GACCAAGAATTGATGAGAT--GACT-CCTG---CTGG-AGTTGAGATGGC---CCCCATG **** ** * * ** *** ** **** **** **** * * ******* hTrex1 125 GTAACTGATCTGCCTTGAGGAAGGAGCCCTGCCCTGCCTGTGGAATTGTCCTGAGTCATT mtrex1 199 GTAACTGATCTGCCCTGAGGAAA-AACCCTGCCCCTCTTCGGGAATTACCCTGAGTCATA ************** ******* * ******** * * ****** ********** hTrex1 185 GCTTTGGGCTGGGGCCATGGGAAGAAACCATTGTGTGGCAGGG--AAGGAGGTGGCTCTT mtrex1 258 GCTTTGGGCGGGGGCCGTGG-----------TG-GGGGCAGGGGTACGTTTGTGGC-CAA ********* ****** *** ** * ******* * * ***** * hTrex1 243 GGCC-CAGGCCTAAACCAGGAAAGCCTGGGAAACTGGGACCCACAGGTGGGCATGAAAGG mtrex1 305 GGAGGCAGCCCTTTTT-AGGAAGGCCTGG--ATCAAGGACTGGGGGAAAGACAAGAAAAG ** *** *** ***** ****** * * **** * * ** **** * hTrex1 302 GCCGCAGCAGGGGCTCCCAGCAGTGTGTAAGACCGGGAGCTGGTCTGGCACCACTGCCCT mtrex1 362 GC------AGAGGCTCACGGGCACATTTGAATCCTAGAG-------------ACAACCCA ** ** ***** * * * * * ** *** ** *** hTrex1 362 GGTCCTTCCAGCTGCCTGTCACTGGTATGATGGCCCCGGTGCATTGTGCCA-CCAGCAGG mtrex1 403 G----TTTGAG-T---TGTCA---GTGTTGGGGCAGTAGTCCATTTTACCAGCCAGCAGG * ** ** * ***** ** * *** ** **** * *** ******** hTrex1 421 C-CACAGCTGTGGATCTTGGAAGGCCTCTGGGGTCCCCCGGGAGCAGGGGAGTGGGTGTG mtrex1 452 ATCACAGATATGGATCTAGGAAGGCCTTTGGGGT---------GCTTGGG-GAGGGAG-- ***** * ******* ********* ****** ** *** * *** * hTrex1 480 GGGGGGAACGGATGGTGGTGAGAGGGACAGACCAGGCAGGCTGACGAGCAGGGCGGGCCT mtrex1 500 -GGAGGAGCGTCAGCA----CGAGGGCCAGGCCTGGCAGGCTAAC-AGCAGGGCGGGCCC ** *** ** * ***** *** ** ******** ** ************* hTrex1 540 GGCTCACGTGG---GCCTGTAGGCGGGCCCACGCCAAGTTTCACTTCC-C-GCCAC--TG mtrex1 554 TGTTCAGGAGGCAGGCCTATGGGTGGACCCAGGC--AGTTTTACTTCCTCAGCCACACTG * *** * ** **** * ** ** **** ** ***** ****** * ***** ** hTrex1 593 CTGCCAGCGAGAGCCGCGGGAGAGTGTGCAGCCGAGTCACTACTGCCTGCCTGCCTGCCT mtrex1 612 CTGCCAGCAGCAGAC-C-----------CAGCGGAGT-------GCCTTGCTGCCTGC-T ******** ** * * **** **** **** ******** * hTrex1 653 GCTACGGTGAGTGTGGCCCCCACAATGGG-ATGGCGCAGGGCAGGAGGGCCATGGGTTCC mtrex1 652 TCT-CGGTAAGTGTGG-----AGTAAGGGTATGGGGTAAGGCAGAAGAGAATAGGGTTCC ** **** ******* * * *** **** * * ***** ** * ******* hTrex1 712 CCCACCCCAGACTAAGGGGGCACTAGGGGAGGGGCCGAGTCATGTGAAGAGGGAGACCCT mtrex1 706 CTCACCCTGAAGGTAGTCAGCACTAGGGGAAGGGCC---TCCC-TCAAAAGGCAAA---- * ***** * ** *********** ***** ** * ** *** * * hTrex1 772 CTCAGACAGTCGAATGTGCTGGTCCCACTAAGGAAACCACCTCACCCTCTCCAACTTCCT mtrex1 758 -TAAGT-AGTGGA---TCCA--TCCCACTA---GAACAACC-CTGCC-CTCCAGCTTCTA * ** *** ** * * ******** *** *** * ** ***** **** hTrex1 832 G----CCTGAAAA mtrex1 806 GTGGCCCTGGATA * **** * * Supplement 4

  5. anti-Trex1 TO-PRO-3 overlay sc8-con wt-con wt-con Supplement 5

  6. A C * * * * Cells (%) Viability (WST, %) UV (J/m2) 12h 24h B con UV nuclear localization (% of cells) 83.1% 224.7% Supplement 6

More Related